Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 8.3 x 7.3 cm

Invitrogen™ Nitrocellulose/Filter Paper Sandwiches are ready-to-use membrane/filter paper assemblies designed for efficient and reliable transfer of proteins and nucleic acids in Western blotting, dot blotting, and hybridization workflows. These sandwiches pair high-performance nitrocellulose membranes with absorbent filter paper in a convenient, standardized format that saves time and improves reproducibility in electrophoretic transfers.

Available in two pore sizes, these membranes cover a wide application range:

• 0.2 μm pore size (LC2000): Optimized for low molecular weight proteins (<20 kDa) and short nucleic acids (<300 bp)
• 0.45 μm pore size (LC2001): Suitable for general protein blotting, especially proteins >20 kDa and larger nucleic acids

Each sandwich is pre-cut to 8.3 × 7.3 cm, ideal for mini gel formats, and delivers high binding capacity with excellent retention of transferred biomolecules.

Key Features and Benefits

• Available in two pore sizes:
  • 0.2 µm (LC2000): For enhanced retention of small proteins and nucleic acids
  • 0.45 µm (LC2001): General-purpose blotting for proteins >20 kDa
• Pre-assembled format – saves prep time and ensures consistent contact
• High binding efficiency (~209 μg/cm²) – captures proteins with high fidelity
• Hydrophobic and electrostatic interactions – promote stable protein binding
• Versatile compatibility – suitable for chemiluminescence, fluorescence, staining, and radiolabeling
• Ready to use – no cutting or assembly required
• Room temperature storage – stable and convenient

Specifications

Applications

• Western blotting (especially small or large molecular weight proteins depending on pore size)
• Nucleic acid hybridization and DNA/RNA transfer
• Dot blot and slot blot assays
• Compatible with detection systems including HRP chemiluminescence, fluorescence, and colorimetric assays

Storage and Handling

• Store at room temperature
• Protect from dust and direct sunlight
• Supplied in airtight, sealed packaging for long-term integrity

The Invitrogen™ Nitrocellulose/Filter Paper Sandwiches offer exceptional performance, convenience, and flexibility for protein and nucleic acid blotting workflows. With both 0.2 µm and 0.45 µm pore sizes available, they support a broad spectrum of analytical needs from low-molecular-weight protein detection to general blotting applications—making them a staple in any molecular biology or proteomics lab.