Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit

The Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit is a comprehensive buffer system designed specifically for the efficient separation of medium to large molecular weight proteins on NuPAGE™ Tris-Acetate Gels. This ready-to-use kit includes four essential reagents needed to prepare and run your SDS-PAGE workflow with confidence, ensuring optimal resolution and protein integrity.

The included buffers and reducing agents support denaturing electrophoresis, minimizing sample degradation and disulfide interference. Together, they offer a complete and optimized electrophoresis solution for researchers working with high-molecular-weight proteins.

Kit Components

Key Features and Benefits

• Complete kit for NuPAGE™ Tris-Acetate SDS-PAGE workflows
• Supports separation of medium-to-large proteins (ideal for >200 kDa)
• Neutral pH system reduces protein degradation and increases resolution
• Includes reducing agent and antioxidant to maintain protein integrity during electrophoresis
• Ready-to-use formats – no additional mixing or pH adjustments needed

Applications

• SDS-PAGE analysis of medium to high molecular weight proteins
• Western blotting and downstream proteomic analysis
• Routine and high-throughput protein size separation
• Sample preparation for mass spectrometry

Compatible Products

• NuPAGE™ Tris-Acetate Precast Gels
• NuPAGE™ Novex Mini Cell & Mini Gel Tank
• iBlot™ 2 or Power Blotter for Western transfer
• NuPAGE™ Prestained Protein Standards

The NuPAGE™ Tris-Acetate SDS Buffer Kit delivers all the essential reagents needed for optimal separation and analysis of larger proteins using SDS-PAGE. With simplified workflow support and reagents formulated to enhance resolution, reduce oxidation, and protect protein structure, this kit is a critical component for researchers demanding precision in protein separation and blotting workflows.