Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

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Specifications:
Application Transfection
Storage Temperature 2-8°C
Product Type Molecular Biology Reagent Forms Kit with Various components
Product Brand Thermo Fisher Scientific™
Product Grade Molecular Biology

Lipofectamine™ 3000 Transfection Reagent represents Invitrogen’s most advanced lipid nanoparticle-based transfection technology, delivering exceptional efficiency, low cytotoxicity, and broad cell compatibility. Designed to perform across both common and hard-to-transfect cell types, it supports reliable delivery of plasmid DNA, RNA, and co-transfection experiments, including genome editing applications with CRISPR/Cas9 or TALENs.

Key Features

  • Unmatched Efficiency: Highest transfection rates across more than 60 cell types, including stem cells, primary cells, and immortalized lines
  • Broad Application Use: Enables DNA, RNA, and multiplex co-transfections in a single protocol
  • Enhanced Cell Viability: Optimized for minimal toxicity, even at high efficiency
  • Versatile Format: Compatible with various plate formats and flask scales (6- to 96-well plates, T-flasks)
  • Cost-Effective: High concentration formulation means fewer microliters needed per reaction

Efficient Across a Range of Cell Types

Lipofectamine 3000 is validated in numerous cell lines:

Cell TypeExamples
FibroblastNIH/3T3, COS-7
MyoblastC2C12, L6
HepatocyteHepG2, HuH7
Epithelial CarcinomaHeLa, A549, MCF7, SK-MEL-28, Caco-2
HematopoieticK562
OsteosarcomaU-2 OS, Saos-2
Colon/ProstateSW480, LNCap
PancreaticPANC-1

Genome Editing Compatibility

Lipofectamine™ 3000 improves transfection success in CRISPR and TALEN workflows by:

  • Increasing nucleic acid uptake
  • Boosting cleavage and recombination efficiency
  • Reducing toxicity that may compromise editing fidelity

Kit Contents and Storage

  • Included: Lipofectamine™ 3000 Reagent and P3000™ Enhancer Reagent
  • Storage: 2–8°C (Do not freeze)
  • Shelf Life: Stable for 12 months from date of manufacture under proper storage conditions

Specifications

AttributeDetails
Product TypeTransfection Reagent
Transfection MethodLipid-based nanoparticle
Target MoleculesPlasmid DNA, siRNA, shRNA, miRNA, mRNA
ApplicationTransfection, Co-transfection, Genome Editing
Cell TypesHard-to-transfect, Stem Cells, Primary, Common Lines
Serum CompatibilityYes
Formats Supported6-, 12-, 24-, 48-, 96-well plates, flasks
ClassificationAnimal Origin-Free (AOF)
SustainabilitySupplied in recyclable, sustainable packaging
Unit Sizes0.1 mL – 15 mL


Lipofectamine™ 3000 offers a breakthrough in transfection reagent technology, delivering higher transfection efficiency, greater reproducibility, and lower cytotoxicity than earlier generations. Its broad versatility and optimized performance make it the ideal solution for high-efficiency DNA, RNA, and genome editing delivery across virtually all cell types.


  • Pack Size:   15 mL 0.1 mL 0.75 mL 1.5 mL 5 x 1.5 mL

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