Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent represents Invitrogen’s most advanced lipid nanoparticle-based transfection technology, delivering exceptional efficiency, low cytotoxicity, and broad cell compatibility. Designed to perform across both common and hard-to-transfect cell types, it supports reliable delivery of plasmid DNA, RNA, and co-transfection experiments, including genome editing applications with CRISPR/Cas9 or TALENs.

Key Features

• Unmatched Efficiency: Highest transfection rates across more than 60 cell types, including stem cells, primary cells, and immortalized lines
• Broad Application Use: Enables DNA, RNA, and multiplex co-transfections in a single protocol
• Enhanced Cell Viability: Optimized for minimal toxicity, even at high efficiency
• Versatile Format: Compatible with various plate formats and flask scales (6- to 96-well plates, T-flasks)
• Cost-Effective: High concentration formulation means fewer microliters needed per reaction

Efficient Across a Range of Cell Types

Lipofectamine 3000 is validated in numerous cell lines:

Genome Editing Compatibility

Lipofectamine™ 3000 improves transfection success in CRISPR and TALEN workflows by:

• Increasing nucleic acid uptake
• Boosting cleavage and recombination efficiency
• Reducing toxicity that may compromise editing fidelity

Kit Contents and Storage

• Included: Lipofectamine™ 3000 Reagent and P3000™ Enhancer Reagent
• Storage: 2–8°C (Do not freeze)
• Shelf Life: Stable for 12 months from date of manufacture under proper storage conditions

Specifications

Lipofectamine™ 3000 offers a breakthrough in transfection reagent technology, delivering higher transfection efficiency, greater reproducibility, and lower cytotoxicity than earlier generations. Its broad versatility and optimized performance make it the ideal solution for high-efficiency DNA, RNA, and genome editing delivery across virtually all cell types.