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Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)
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Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ GlycoBlue™ Coprecipitant is a highly effective reagent designed to aid in the recovery of nucleic acids during alcohol precipitation, particularly when working with low concentrations of DNA or RNA. It combines glycogen—a branched carbohydrate polymer—with a covalently linked blue dye, providing both quantitative nucleic acid recovery and easy visualization of pellets after centrifugation.
With a stock concentration of 15 mg/mL, GlycoBlue is ideal for applications such as RT-PCR, nuclease protection assays, and other workflows where nucleic acid recovery at ng/mL concentrations is required. Its use minimizes sample loss and prevents invisible pellets during precipitation.
Key Features
- Enhanced Visibility – Blue dye improves pellet detection during alcohol precipitation.
- High Recovery Efficiency – Enables quantitative recovery of nucleic acids from dilute solutions.
- Reliable Performance – Does not interfere with enzymatic reactions, making it superior to yeast RNA as a coprecipitant.
- Nuclease-Free – Treated with Proteinase K and SDS, then purified to ensure no RNase or DNase activity.
- Convenient Formats – Available in 300 μL or 5 × 300 μL tubes.
Applications
- RT-PCR and qPCR workflows.
- Nuclease protection assays.
- Northern blotting and hybridization.
- Recovery of RNA or DNA from low-concentration or dilute samples.
- Visualization aid in precipitation-based workflows.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Nucleic Acid Coprecipitant |
| Concentration | 15 mg/mL |
| Form | Liquid |
| Catalog Numbers | AM9515 (300 μL), AM9516 (5 × 300 μL) |
| Final Use Concentration | 50–150 μg/mL |
| Purity | Molecular biology grade, RNase- and DNase-free |
| Pellet Recovery | Efficient, visible blue pellet |
| Product Line | Ambion™, GlycoBlue™ |
| Shipping Condition | Dry ice |
| Storage | –20 °C |
| Unit Size | Each tube |
Unique Advantages
- Ensures reproducible results when working with trace nucleic acid levels.
- Offers visual confirmation of pellet presence, reducing loss during wash steps.
- Preferred over yeast RNA coprecipitants, as it does not add interfering nucleic acids.
- Widely trusted in molecular biology, virology, and genomics laboratories.
The Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL) is a robust solution for improving nucleic acid recovery during alcohol precipitation. By combining glycogen with a blue dye, it delivers both high recovery efficiency and easy pellet visualization, ensuring reliable performance in sensitive workflows such as RT-PCR, nuclease protection assays, and sequencing preparation.
- Pack Size: 300 μl 5 x 300 μL