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Invitrogen™ PureLink™ RNA Mini Kit
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Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
Specifications:
| Application | Genomic Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ RNA Mini Kit provides a fast and reliable method for isolating high-quality total RNA from diverse biological samples, including mammalian tissues, cultured cells, yeast, bacteria, blood, and plant material. Utilizing a silica spin column-based purification system, the kit enables RNA recovery in as little as 20 minutes, with no need for hazardous chemicals or time-intensive protocols.
With an extra-large binding capacity, the kit supports RNA isolation from up to 200 mg of tissue or 10⁸ cells, yielding up to 1000 μg of total RNA—significantly more than most mini kits on the market. Purified RNA is compatible with a variety of downstream applications including qPCR, RT-PCR, Northern blotting, NGS, and microarray analysis.
Key Features and Benefits
- Fast protocol – Isolate total RNA in ~20 minutes
- High RNA yield – Recover up to 1000 μg per prep
- Flexible input range – Works with 5–200 mg of tissue or 5 × 10⁶ to 10⁸ cells
- Minimal DNA carryover – Optional DNase treatment available for DNA-sensitive applications
- Safe and efficient – No phenol/chloroform extraction required
- Versatile sample compatibility – Works with animal, plant, microbial, and enzymatic samples
- Low elution volumes – Recover RNA in as little as 30 μL
- RNase-free – Includes certified RNase-free buffers and spin columns
Applications
- Gene expression analysis (RT-PCR, qPCR)
- Microarray analysis
- RNA sequencing (RNA-Seq, NGS)
- Northern blotting and hybridization
- cDNA library construction
- In vitro transcription/translation
- Nuclease protection assays
- RNA labeling
Ordering Information
| Catalog Number | Quantity | Description |
|---|---|---|
| 12183020 | 10 preps | Mini prep format |
| 12183018A | 50 preps | Standard prep kit |
| 12183025 | 250 preps | Bulk RNA isolation kit |
Optional accessory:
- PureLink™ DNase Set (Cat. No. 12185010) – for on-column or post-purification DNA removal
- PureLink™ Homogenizer (Cat. No. 12183026) – for plant or tough tissue homogenization
Specifications
| Attribute | Details |
|---|---|
| Final Product | Total RNA |
| Sample Types | Cells, tissues, blood, bacteria, yeast, plant, enzymes |
| Yield | Up to 1000 μg per prep (typical: up to 350 μg) |
| Elution Volume | 30–300 μL |
| Purification Time | ~20 minutes (post-homogenization) |
| Starting Material | Tissue (≤200 mg), Cells (≤5 × 10⁷), Yeast (≤5 × 10⁸), Blood (≤0.2 mL), Bacteria (≤10⁹), Enzyme reactions (≤1.2 mL) |
| Scale | Mini |
| Format | Silica spin column |
| Compatibility | Manual (not for high-throughput automation) |
| Storage | Room temperature |
| Shelf Life | Refer to individual component labels |
Kit Contents
- Lysis Buffer
- Wash Buffer I
- Wash Buffer II
- RNase-Free Water
- Spin Cartridges with Collection Tubes
- Recovery Tubes
The Invitrogen™ PureLink™ RNA Mini Kit is a trusted and efficient solution for isolating high-quality total RNA across a wide range of sample types. Its fast workflow, large binding capacity, and minimal DNA contamination make it an excellent choice for molecular biology labs performing gene expression profiling, transcriptome analysis, and RNA-based assays.
- Reactions: 50 rxn 250 rxn 10 rxn