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Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina™ Systems
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Specifications:
| Application | Next Generation Sequencing | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | NGS library Prep kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina™ Systems is a complete, high-performance solution designed for rapid and reliable preparation of sequencing-ready DNA libraries for Illumina next-generation sequencing (NGS) platforms. This kit supports a wide range of DNA inputs—including fragmented genomic DNA, cell-free DNA (cfDNA), and short amplicons—and delivers superior coverage uniformity and exceptional variant detection accuracy.
Powered by optimized enzymatic reactions, magnetic bead-based purification, and high-fidelity amplification chemistry, the Collibri PS DNA Library Prep Kit enables consistent, reproducible library preparation with minimal bias. The workflow is optimized for automation, multiplex sequencing, and PCR or PCR-free protocols, making it ideal for research laboratories, sequencing core facilities, and genomics centers.
Key Features and Benefits
High-Confidence Variant Detection
- Enables accurate identification of SNPs, INDELs, and structural variants
- Improved coverage across GC-rich and complex genomic regions
- Suitable for high-precision genomic analysis
Flexible DNA Input Range
- Compatible with input DNA from 1 ng to 1 μg
- Supports fragmented DNA, cfDNA, and amplicons (≤300 bp)
- PCR-free workflow option available for ≥500 ng input DNA
Fast and Efficient Workflow
- Library preparation completed in approximately 3 hours
- Reduced turnaround time for sequencing workflows
- Minimal hands-on time
Multiplexing Capability
- Supports single-index and unique dual-index adapters
- Enables multiplexing of up to 96 samples
- Minimizes index hopping and improves sequencing accuracy
Automation-Ready Workflow
- Compatible with automated liquid handling systems
- Ideal for high-throughput sequencing laboratories
Visual Workflow Tracking
- Built-in visual indicator dyes assist pipetting accuracy
- Enhances workflow reliability and reduces errors
Optimized for Illumina Sequencing Platforms
Compatible with:
- NovaSeq™ 6000
- NextSeq™ 1000 & 2000
- NextSeq™ 550
- MiSeq™
- HiSeq™ Series
- iSeq™ Systems
Applications
The Collibri PS DNA Library Prep Kit is ideal for:
- Whole genome sequencing (WGS)
- Targeted sequencing
- Resequencing studies
- De novo genome assembly
- Cancer genomics research
- Cell-free DNA sequencing
- Microbial genome sequencing
- Population genomics
- Precision medicine research
Kit Variants and Configurations
The Collibri PS DNA Library Prep Kit is available in multiple configurations to meet diverse workflow requirements, including core kits, kits with cleanup modules, and kits with single or dual indexes.
| Catalog Number | No. of Preps | Kit Configuration | Kit Contents | Storage Temperature |
|---|---|---|---|---|
| A38612024W | 24 | Core Kit | PS DNA Library Prep Kit, Cleanup Kit | Prep Kit: −20°C Cleanup Kit: 4°C |
| A38614096W | 96 | Core Kit | PS DNA Library Prep Kit, Cleanup Kit | Prep Kit: −20°C Cleanup Kit: 4°C |
| A38612024 | 24 | Library Prep Kit with CD Indexes | PS DNA Library Prep Kit, Cleanup Kit, DNA CD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A38614096 | 96 | Library Prep Kit with CD Indexes | PS DNA Library Prep Kit, Cleanup Kit, DNA CD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A38613024 | 24 | Kit with UD Indexes Set A (1–24) | PS DNA Library Prep Kit, Cleanup Kit, DNA UD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A43611024 | 24 | Kit with UD Indexes Set B (25–48) | PS DNA Library Prep Kit, Cleanup Kit, DNA UD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A43612024 | 24 | Kit with UD Indexes Set C (49–72) | PS DNA Library Prep Kit, Cleanup Kit, DNA UD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A43613024 | 24 | Kit with UD Indexes Set D (73–96) | PS DNA Library Prep Kit, Cleanup Kit, DNA UD Indexes | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
| A38614196 | 96 | Kit with UD Indexes Sets A–D (1–96) | PS DNA Library Prep Kit, Cleanup Kit, Complete DNA UD Index Sets | Prep Kit: −20°C Cleanup Kit: 4°C Indexes: −20°C |
Legend:
CD = Combinatorial Dual Indexes
UD = Unique Dual Indexes
Workflow Overview
Typical workflow steps include:
- DNA fragmentation (if required)
- End repair and adapter ligation
- Cleanup and size selection
- PCR amplification or PCR-free workflow
- Index incorporation
- Generation of sequencing-ready libraries
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | DNA Library Preparation Kit |
| Input DNA Range | 1 ng to 1 μg |
| Workflow Time | ~3 hours |
| Sequencing Compatibility | Illumina Platforms |
| Library Type | Fragment Library |
| Multiplexing Capability | Up to 96 samples |
| Automation Compatibility | Yes |
| Sample Type | Genomic DNA, cfDNA, Amplicons |
| Storage | −20°C and 4°C depending on module |
| Format | Complete Kit |
Advantages Over Conventional Library Preparation Kits
- Faster workflow with minimal hands-on time
- Higher sequencing accuracy and reproducibility
- Superior genome coverage uniformity
- Flexible indexing and multiplexing options
- PCR and PCR-free workflows available
- Automation compatible for high throughput labs
The Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina™ Systems delivers a fast, reliable, and high-precision solution for DNA library preparation in modern genomics workflows. With flexible configurations, high-fidelity amplification, automation compatibility, and superior sequencing performance, this kit enables laboratories to generate high-quality sequencing libraries with confidence, making it an essential tool for whole genome sequencing, targeted sequencing, and advanced genomic research.
- Kit Content Configuration: PS DNA Library Prep Kit + Cleanup Kit (24 Preps) PS DNA Library Prep Kit + Cleanup Kit (96 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA CD Indexes (24 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA CD Indexes (96 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA UD Indexes Set A (1–24) (24 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA UD Indexes Set B (25–48) (24 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA UD Indexes Set C (49–72) (24 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA UD Indexes Set D (73–96) (24 Preps) PS DNA Library Prep Kit + Cleanup Kit + DNA UD Indexes Sets A–D (1–96) (96 Preps)