Invitrogen™ CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry

Bright, stable, and reliable dye for tracking cell proliferation in vitro and in vivo

The Invitrogen™ CellTrace™ Far Red Cell Proliferation Kit provides a robust and sensitive solution for labeling cells to monitor multiple generations by dye dilution using flow cytometry. Designed for both in vitro and in vivo applications, this kit enables precise tracking of cell division over several days, with minimal cytotoxic effects and strong, uniform fluorescence.

With red excitation at 630 nm and emission at 661 nm, the dye offers limited spectral overlap, making it ideal for multiplexing with common fluorophores such as Alexa Fluor™ 488, FITC, RPE, and fluorescent proteins including GFP and mCherry.

Key Features & Benefits

• High Sensitivity – Bright, single-peak fluorescence allows visualization of six or more generations before signal loss to auto-fluorescence.
• Long-Term Stability – Covalently binds to intracellular and surface amines for consistent signal retention over several days.
• Low Cytotoxicity – Minimal impact on cell viability and proliferation.
• Multiplex-Compatible – Minimal spectral overlap with other common dyes and fluorescent proteins.
• User-Friendly Protocol – Single-use vials for small-scale experiments and consistent results.

Applications

• Cell Proliferation Studies – Monitor multiple cell generations in culture or animal models.
• Immune Cell Tracking – Follow division patterns of T cells, B cells, or other immune cell subsets.
• Drug Screening – Evaluate proliferation effects of therapeutic compounds.
• Stem Cell Research – Assess proliferation capacity and cell lineage tracking.

Technical Specifications

Kit Contents

• 1 vial CellTrace™ Far Red dye
• 1 vial DMSO (100 μL)

Ordering Information

The Invitrogen™ CellTrace™ Far Red Cell Proliferation Kit offers researchers a reliable, high-sensitivity, and stable method for tracking cell division across multiple generations. Its low cytotoxicity, long-term signal retention, and multiplexing capabilities make it ideal for complex flow cytometry experiments in immunology, oncology, and stem cell research.