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Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 670
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | –80°C | ||
| Product Type | Flow Cytometry Reagents | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Red Fluorescent Dye for Tracking Cell Division in Flow Cytometry and Microscopy
Cell Proliferation Dye eFluor™ 670 is a red-emitting amine-reactive dye used to monitor individual cell divisions and track cell populations over time via flow cytometry or fluorescence microscopy. The dye covalently binds to primary amines on intracellular and membrane proteins, and upon cell division, is equally distributed between daughter cells—resulting in sequential halving of fluorescence intensity that allows up to 6 generations to be distinguished.
With excitation at 647 nm and emission at 670 nm, eFluor™ 670 is compatible with red (633 nm) lasers and can be detected in the 660/20 bandpass channel (equivalent to APC, Alexa Fluor™ 647, and eFluor™ 660), making it ideal for use alongside GFP and other commonly used fluorophores.
Key Features and Benefits
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Reliable Cell Division Tracking
Allows clear visualization of up to 6 cell divisions based on dye dilution. -
Compatible with Fixation and Permeabilization
Labeled cells can be fixed and permeabilized for intracellular staining without loss of fluorescence. -
Spectral Compatibility
Excitable by 633–647 nm lasers with minimal overlap with GFP, enabling use in multiparameter panels. -
Long-Term Tracking
Supports long-term cell fate mapping, clonal expansion, and in vivo tracking studies. -
Stable and Versatile Format
Supplied as a lyophilized powder; reconstitutes to 5 mM stock in DMSO for flexible use.
Applications
- Proliferation tracking in flow cytometry
- T cell and B cell clonal expansion assays
- Stem cell fate and division history studies
- Longitudinal in vivo cell tracking
- Microscopy-based division analysis
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Cell Proliferation Dye eFluor™ 670 |
| Catalog Numbers | 65-0840-85 (500 µg), 65-0840-90 (4 × 500 µg) |
| Form | Lyophilized powder |
| Molecular Weight | 792.6 g/mol |
| Reconstitution | 5 mM stock solution with 126 µL anhydrous DMSO |
| Excitation / Emission | 647 nm / 670 nm (detect using 660/20 filter) |
| Color | Red |
| Detection Method | Fluorescence |
| Compatible Equipment | Flow Cytometer, Fluorescence Microscope |
| Fixation-Compatible | Yes (with formaldehyde & saponin-based buffers) |
| Storage Conditions | ≤ –20°C with desiccant, protect from light |
| Stability | Use within 6 months of reconstitution; avoid freeze-thaw |
| Unit Size | Each (500 µg) |
| Product Line | eBioscience™ |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 670 provides a robust and reliable solution for tracking cell division history in flow cytometry and microscopy. With fixation compatibility, bright red fluorescence, and minimal interference with commonly used fluorophores, it's an ideal tool for immunology, cancer biology, and stem cell research.
- Pack Size: 500 μg 4 x 500 μg