Invitrogen™ CellEvent™ Senescence Green Detection Kit

Fluorescent β-galactosidase-based probe for sensitive detection of cellular senescence

The Invitrogen™ CellEvent™ Senescence Green Detection Kit provides a highly sensitive, fluorescence-based method for the image-based detection of senescent mammalian cells. This kit includes a fluorescein-based probe containing two galactoside moieties, making it a specific target for β-galactosidase, a biomarker strongly associated with cellular senescence.

Upon enzymatic cleavage by β-galactosidase, the probe generates a covalently bound, cell-retained fluorescent product with excitation/emission maxima of 490/514 nm, easily detected with standard Alexa Fluor™ 488/FITC filter sets. The probe’s fluorescence is well retained, enabling clear imaging and analysis over extended periods.

Designed for use with fixed samples, the kit’s optimized buffer and compatibility with paraformaldehyde fixation allow multiplexing with other fluorescent reagents, making it an excellent choice for high-content analysis and fluorescence microscopy workflows.

Key Features

• Highly Sensitive Detection – Greater sensitivity than traditional colorimetric x-gal assays
• Fast and Reliable – Generates reproducible, robust fluorescence signals
• Multiplex-Compatible – Works alongside other paraformaldehyde-compatible fluorescent markers
• Long-Term Signal Retention – Cleaved product binds covalently to intracellular proteins
• Standard Equipment Compatible – Detectable with common FITC/Alexa Fluor™ 488 filter sets

Applications

• Detection of cellular senescence in mammalian cells
• High-content imaging of senescent cell populations
• Multiparametric analysis of senescence biomarkers
• Studies in tumor suppression, aging, and tissue repair
• Screening for compounds affecting senescence pathways

Specifications

The CellEvent™ Senescence Green Detection Kit offers a sensitive, specific, and multiplex-capable solution for senescence research. With improved performance over traditional x-gal assays, it enables precise detection of β-galactosidase activity and streamlined integration into high-content imaging workflows.