Agilent 4200 TapeStation System

The Agilent 4200 TapeStation System is a high-throughput, fully automated electrophoresis platform designed for DNA and RNA sample quality control (QC) in next-generation sequencing (NGS), biobank workflows, and molecular biology applications. It provides scalable, user-independent analysis of 1 to 96 samples per run with constant cost per sample, offering high flexibility and efficiency for labs processing variable sample numbers.

With ready-to-use ScreenTape technology, the 4200 TapeStation enables rapid, reproducible assessment of nucleic acid integrity, sizing, and concentration, making it an essential tool for automated, high-throughput DNA and RNA quality control.

Key Features & Benefits

1. Fully Automated Sample Processing with Minimal Hands-On Time
   • Enables unattended analysis of 1 to 96 DNA/RNA samples.
   • Provides user-independent results, eliminating manual variability.
2. Consistent Per-Sample Cost with Scalable Throughput
   • Analyzes any sample number between 1 and 96 without increasing costs.
   • Offers high flexibility for small and large batches.
3. Dual Compatibility for DNA & RNA Quality Control
   • Easily switch between DNA and RNA ScreenTape assays.
   • Ensures precise integrity assessment using DIN (DNA Integrity Number) and RINe (RNA Integrity Number equivalent).
4. Fast, High-Throughput Electrophoresis with Reproducible Results
   • Delivers results in 1–2 minutes per sample, processing 96 samples in under 90 minutes.
   • Provides high precision in fragment sizing, concentration, and integrity assessment.
5. No Cross-Contamination with ScreenTape Technology
   • Each sample is processed in a separate lane, eliminating carryover concerns.
   • Uses disposable loading tips for contamination-free workflows.
6. Low Sample Input & High Sensitivity Analysis
   • Requires only 1–2 µL of DNA or RNA per sample.
   • Works with low-concentration samples while maintaining accuracy.
7. Compact & Low Maintenance Design
   • Features easy self-maintenance tasks to reduce downtime.
   • Offers lowest cost of ownership compared to alternative systems.

Product Specifications

Use Cases & Applications

1. Next-Generation Sequencing (NGS) Library Quality Control

• Ensures High-Quality DNA/RNA for NGS Library Prep
  • Measures RINe for RNA integrity and DIN for DNA fragmentation assessment.
  • Detects adapter dimers, incomplete ligation products, and low-yield libraries.
• Automated Electrophoresis for High-Throughput NGS Pipelines
  • Provides reproducible QC data for sample selection and library validation.
  • Reduces workflow bottlenecks in sequencing labs.

2. Genomic & Cell-Free DNA Integrity Assessment

• Genomic DNA Quality Control for Whole-Genome Sequencing
  • Measures DNA fragmentation patterns with DIN scoring.
  • Prevents degraded or low-quality DNA from impacting sequencing runs.
• cfDNA & Liquid Biopsy Analysis for Oncology Research
  • Supports quality assessment of circulating DNA (cfDNA) and tumor-derived DNA.
  • Provides precise fragment size distribution for biomarker studies.

3. RNA Quality Control for Gene Expression & Transcriptomics

• Determines RNA Integrity for qPCR & Microarrays
  • Rapidly assesses total RNA and mRNA quality for transcriptomics research.
  • Ensures RNA integrity before downstream analysis.
• Optimized for Single-Cell RNA Sequencing (scRNA-Seq)
  • Validates RNA input quality for single-cell applications.
  • Detects partial RNA degradation in low-yield samples.

4. Clinical Research & Biopharmaceutical Development

• Reliable Sample QC for Drug Discovery & Cell Therapy Research
  • Facilitates standardized RNA/DNA analysis in regulated environments.
  • Supports assay development in molecular diagnostics.
• Essential for CRISPR & Genome Editing Workflows
  • Enables precise DNA quality assessment for gene-editing efficiency.
  • Ensures accurate genomic DNA integrity in CRISPR-based studies.

Instructions for Use

1. Load Samples & ScreenTape Device:
   • Place 1 to 96 samples in the TapeStation sample tray.
   • Insert the appropriate DNA or RNA ScreenTape cartridge.
2. Run the TapeStation Analysis:
   • Start the automated electrophoresis process via software.
   • The system automatically processes samples in 1–2 minutes per sample.
3. Interpret Data & Export Results:
   • View electropherogram traces & gel-like images for fragment size analysis.
   • Export results in PDF, CSV, or LIMS-compatible formats.

Storage & Handling Instructions

• Storage Conditions:
  • Store the instrument in a temperature-controlled laboratory.
  • Keep ScreenTape reagents at recommended conditions (4°C or RT, depending on assay type).
• Handling Precautions:
  • Follow standard lab safety protocols when handling nucleic acid samples.
  • Ensure proper calibration and maintenance for reproducible results.
• Disposal Guidelines:
  • Dispose of used ScreenTape cartridges and reagents as per biosafety waste regulations.

Why Choose the Agilent 4200 TapeStation System?

✅ Fully automated DNA/RNA integrity analysis with minimal hands-on time

✅ Flexible throughput (1 to 96 samples) with constant per-sample cost

✅ Proven ScreenTape technology for high reproducibility & accuracy

✅ Fast electrophoresis results (1–2 minutes per sample)

✅ No cross-contamination with individual sample lanes & loading tips

The Agilent 4200 TapeStation System is an advanced electrophoresis platform designed for high-throughput, automated nucleic acid QC, ensuring precise DNA/RNA quality control for NGS, qPCR, and biobank workflows.