Agilent High Sensitivity DNA kit

The Agilent High Sensitivity DNA Kit is a comprehensive microfluidics-based solution for the precise separation, sizing, and quantification of double-stranded DNA (dsDNA) samples ranging from 50 to 7000 base pairs (bp). Designed for use with Agilent Bioanalyzer Systems, this kit provides high-resolution analysis of low-abundance DNA samples, making it ideal for next-generation sequencing (NGS) library QC, PCR fragment validation, and genomic DNA integrity assessment.

This kit includes 10 microfluidic chips, reagents, and consumables, ensuring reliable, high-sensitivity DNA quality control with minimal sample input.

Key Features & Benefits

1. High-Sensitivity Analysis for Low-Abundance DNA
   • Detects dsDNA concentrations as low as 5 pg/µL.
   • Provides accurate quantification and fragment size distribution.
2. Wide Dynamic Range (50–7000 bp)
   • Enables comprehensive analysis of a broad range of DNA fragment sizes.
   • Ideal for quality control of PCR amplicons, genomic DNA, and sequencing libraries.
3. Automated, Microfluidics-Based DNA QC
   • Eliminates manual gel preparation and variability.
   • Ensures high-throughput, reproducible DNA integrity assessment.
4. Complete Kit with Ready-to-Use Components
   • Includes 10 microfluidic chips, sample reagents, and consumables.
   • Pre-measured reagents minimize preparation time and handling errors.
5. Compatible with Agilent Bioanalyzer Systems
   • Seamlessly integrates into automated DNA quality control workflows.
   • Provides electropherogram traces and digital gel-like images for data analysis.

Product Specifications

Use Cases & Applications

1. Next-Generation Sequencing (NGS) Library Quality Control

• Ensures High-Quality DNA Input for NGS Workflows
  • Confirms library fragment size distribution and integrity.
  • Detects adapter dimers, incomplete ligation products, and sample degradation.
• Prevents NGS Sequencing Failures Due to Poor Library Prep
  • Ensures accurate DNA fragmentation analysis for optimal sequencing performance.
  • Detects low-yield libraries with insufficient amplification products.

2. PCR Product Validation & Amplicon Size Determination

• Confirms PCR Fragment Sizes for Cloning & Gene Editing
  • Differentiates specific amplicons from non-specific PCR byproducts.
  • Ensures accurate product validation for multiplex PCR and genotyping assays.
• Sensitive DNA Detection for Low-Input PCR Samples
  • Detects low-abundance DNA fragments in forensic and environmental DNA research.
  • Ideal for qPCR and endpoint PCR quality control.

3. Genomic DNA Integrity & Degradation Analysis

• Monitors DNA Fragmentation in Experimental Samples
  • Evaluates DNA degradation levels for molecular biology workflows.
  • Ensures high-quality genomic DNA for long-read sequencing applications.
• Cell-Free DNA (cfDNA) & Circulating Tumor DNA (ctDNA) Analysis
  • Facilitates high-sensitivity detection of short DNA fragments from liquid biopsies.
  • Used in epigenetics, cancer research, and rare variant detection.

4. Epigenetics & Low-Input DNA Research

• Ideal for Bisulfite Sequencing & Methylation Studies
  • Provides accurate fragment analysis for epigenetic modifications.
  • Detects DNA degradation caused by bisulfite treatment.
• Facilitates Cancer Genomics & Tumor DNA Analysis
  • Ensures DNA quality control for detecting tumor-derived DNA fragments.
  • Used in high-sensitivity assays for rare variant detection.

Instructions for Use

Step-by-Step Protocol for Bioanalyzer Analysis

1. Prepare Samples & Reagents:
   • Warm all reagents and allow to equilibrate to room temperature.
   • Dilute DNA samples if required.
2. Load the Bioanalyzer System:
   • Insert a High Sensitivity DNA microfluidic chip into the Bioanalyzer.
   • Add 1 µL of DNA sample per well.
3. Start the Bioanalyzer Run:
   • Select the High Sensitivity DNA Assay in the software.
   • The system will automatically process samples within ~30 minutes.
4. Analyze & Export Data:
   • View electropherogram traces and gel-like images for DNA fragment analysis.
   • Export data as PDF, CSV, or LIMS-compatible formats.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 4–25°C, protected from light.
  • Do not freeze, as it may affect reagent performance.
• Handling Precautions:
  • Use aseptic techniques when handling DNA samples.
  • Ensure reagents remain sealed until use to prevent contamination.
• Disposal Guidelines:
  • Dispose of used reagents as per laboratory biosafety regulations.
  • Follow institutional waste management protocols for electrophoresis consumables.

Why Choose Agilent High Sensitivity DNA Kit?

✅ Highly sensitive detection for DNA fragments from 50–7000 bp

✅ Automated, microfluidics-based electrophoresis for consistent QC

✅ Preloaded reagents for minimal hands-on time & error-free workflow

✅ Detects low-abundance DNA with as little as 5 pg/µL input

✅ Compatible with Agilent Bioanalyzer for scalable sample processing

The Agilent High Sensitivity DNA Kit is an essential tool for DNA quality control in genomics, sequencing, and molecular biology applications, ensuring high-precision DNA fragment analysis with minimal sample handling.