0
Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL), 100 Units
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Endonuclease IV (Endo IV) is a recombinant DNA repair enzyme derived from E. coli, specialized in recognizing and processing apurinic/apyrimidinic (AP) sites in double-stranded DNA. By cleaving the phosphodiester bond 5′ to AP lesions, it generates a 3′-hydroxyl group suitable for DNA polymerase extension, making it a key tool for DNA repair studies.
In addition to AP endonuclease activity, Endo IV functions as a 3′-diesterase, removing damaged 3′-blocking groups such as 3′-phosphoglycolate and 3′-phosphate. It also exhibits 3′→5′ exonuclease activity, preferentially processing substrates with 3′-recessed ends. The enzyme operates independently of Mg²⁺ and remains active in the presence of moderate EDTA concentrations, ensuring flexibility in diverse experimental conditions.
Key Features
- AP Site Recognition & Cleavage – Specifically cleaves at apurinic/apyrimidinic sites in dsDNA.
- Multi-Activity Enzyme – Functions as an AP endonuclease, 3′-diesterase, and 3′→5′ exonuclease.
- Damage Processing – Removes 3′-phosphoglycolate and 3′-phosphate groups from damaged DNA ends.
- Metal-Independent – No Mg²⁺ required; remains active even in the presence of EDTA.
- Research-Grade Purity – Suitable for high-precision DNA damage and repair studies.
Applications
- DNA damage and repair research
- Single-cell electrophoresis (comet assay)
- Antitumor drug mechanism studies
- DNA structure and repair pathway analysis
- SNP analysis and mutation detection
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Endonuclease IV |
| Catalog Number | EN0591 |
| Quantity | 100 units |
| Concentration | 2 U/µL |
| Enzyme | Endonuclease IV (E. coli) |
| Activities | AP endonuclease, 3′-diesterase, 3′→5′ exonuclease |
| Substrate Specificity | AP sites in dsDNA; damaged 3′-ends (phosphoglycolate, phosphate) |
| Preferred Substrates | dsDNA with 3′-recessed ends |
| Metal Requirement | None (Mg²⁺ not required) |
| EDTA Sensitivity | Active in moderate EDTA concentrations |
| Unit Size | Each |
| Storage Conditions | –20 °C |
| Shipping Condition | Dry ice |
Ordering Information
| Catalog Number | Quantity | Concentration | Unit Size |
|---|---|---|---|
| EN0591 | 100 units | 2 U/µL | Each |
The Thermo Scientific™ Endonuclease IV (Endo IV) is a versatile, multi-functional DNA repair enzyme with AP endonuclease, 3′-diesterase, and 3′→5′ exonuclease activities. Its ability to process damaged DNA ends and function without Mg²⁺ makes it invaluable in DNA repair studies, SNP analysis, comet assays, and antitumor drug research. With robust activity and high specificity, it is an essential reagent for genomic stability and DNA repair pathway investigations.