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The Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human (ab252190) is a multiplex antibody sampler kit designed for the reliable detection of key DNA mismatch repair (MMR) proteins by immunohistochemistry (IHC) and other applications. The panel includes four high-quality recombinant rabbit monoclonal antibodies against MSH6, PMS2, MLH1, and MSH2, each validated in knockout models to ensure target specificity.
These proteins are essential in the DNA mismatch repair pathway, where they safeguard genomic integrity by correcting replication errors and DNA damage. Defects in MMR proteins are strongly associated with microsatellite instability (MSI) and cancers such as Lynch syndrome (HNPCC) and Muir-Torre Syndrome (MTS). This antibody panel provides a cost-effective, trial-sized solution for laboratories performing MMR IHC profiling in cancer research.
Key Features & Benefits
- Comprehensive MMR Coverage – Targets four critical mismatch repair proteins: MSH6, PMS2, MLH1, MSH2.
- Knockout Validated – Each antibody is validated for specificity using KO models.
- Optimized for IHC – Excellent performance in FFPE tissue immunohistochemistry.
- Recombinant Rabbit Monoclonals – High batch-to-batch consistency and sensitivity.
- Trial-Sized Format – Convenient volumes (20 µL each) for assay evaluation.
- Pathology-Relevant – Supports detection of MMR protein loss in cancer research (e.g., colorectal, endometrial, ovarian cancers).
Included Antibodies
| Catalog Number | Target Protein | Clone | Volume |
|---|---|---|---|
| ab92312 | MLH1 | [EPR3894] | 2 × 10 µL |
| ab227941 | MSH2 | [EPR21017-123] | 2 × 10 µL |
| ab92471 | MSH6 | [EPR3945] | 2 × 10 µL |
| ab110638 | PMS2 | [EPR3947] | 2 × 10 µL |
Applications
- Immunohistochemistry (IHC-P) – Detection of MMR proteins in formalin-fixed, paraffin-embedded tissue.
- Immunocytochemistry / Immunofluorescence (ICC/IF) – Cellular localization of mismatch repair proteins.
- Cancer Research – Evaluation of MMR deficiency and MSI in colorectal, endometrial, and other cancers.
- Molecular Pathology Studies – Research on DNA repair, genomic stability, and apoptosis regulation.
Product Specifications
| Attribute | Detail |
|---|---|
| Product Code | ab252190 |
| Reactivity | Human |
| Host Species | Rabbit |
| Antibody Type | Recombinant monoclonal |
| Form | Liquid |
| Conjugate | Unconjugated |
| Storage | Shipped on blue ice; follow antibody-specific protocols for long-term storage |
| Quantity | 1 kit (4 antibodies, trial-size) |
| Intended Use | For research use only |
Scientific Context
- MMR Function – MSH2 pairs with MSH6 to form MutSα, while MLH1 pairs with PMS2 to form MutLα. Together, these complexes detect and repair mismatches in DNA, preventing the accumulation of mutations.
- Cancer Relevance – Defective MMR proteins cause microsatellite instability (MSI), a hallmark of Lynch syndrome and associated malignancies (colorectal, gastric, endometrial).
- Pathway Links – MMR proteins interact with DNA replication machinery, p53 signaling, and apoptosis pathways, making them critical biomarkers for cancer research.
The Abcam Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human (ab252190) is a powerful, validated toolkit for researchers investigating DNA repair mechanisms, genomic stability, and cancer biomarkers. With four high-performance recombinant rabbit monoclonal antibodies, this panel supports robust IHC and ICC/IF workflows for mismatch repair protein profiling in FFPE tissues and human cell samples.
Datasheet
The Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human (ab252190) is a multiplex antibody sampler kit designed for the reliable detection of key DNA mismatch repair (MMR) proteins by immunohistochemistry (IHC) and other applications. The panel includes four high-quality recombinant rabbit monoclonal antibodies against MSH6, PMS2, MLH1, and MSH2, each validated in knockout models to ensure target specificity.
These proteins are essential in the DNA mismatch repair pathway, where they safeguard genomic integrity by correcting replication errors and DNA damage. Defects in MMR proteins are strongly associated with microsatellite instability (MSI) and cancers such as Lynch syndrome (HNPCC) and Muir-Torre Syndrome (MTS). This antibody panel provides a cost-effective, trial-sized solution for laboratories performing MMR IHC profiling in cancer research.
Key Features & Benefits
- Comprehensive MMR Coverage – Targets four critical mismatch repair proteins: MSH6, PMS2, MLH1, MSH2.
- Knockout Validated – Each antibody is validated for specificity using KO models.
- Optimized for IHC – Excellent performance in FFPE tissue immunohistochemistry.
- Recombinant Rabbit Monoclonals – High batch-to-batch consistency and sensitivity.
- Trial-Sized Format – Convenient volumes (20 µL each) for assay evaluation.
- Pathology-Relevant – Supports detection of MMR protein loss in cancer research (e.g., colorectal, endometrial, ovarian cancers).
Included Antibodies
| Catalog Number | Target Protein | Clone | Volume |
|---|---|---|---|
| ab92312 | MLH1 | [EPR3894] | 2 × 10 µL |
| ab227941 | MSH2 | [EPR21017-123] | 2 × 10 µL |
| ab92471 | MSH6 | [EPR3945] | 2 × 10 µL |
| ab110638 | PMS2 | [EPR3947] | 2 × 10 µL |
Applications
- Immunohistochemistry (IHC-P) – Detection of MMR proteins in formalin-fixed, paraffin-embedded tissue.
- Immunocytochemistry / Immunofluorescence (ICC/IF) – Cellular localization of mismatch repair proteins.
- Cancer Research – Evaluation of MMR deficiency and MSI in colorectal, endometrial, and other cancers.
- Molecular Pathology Studies – Research on DNA repair, genomic stability, and apoptosis regulation.
Product Specifications
| Attribute | Detail |
|---|---|
| Product Code | ab252190 |
| Reactivity | Human |
| Host Species | Rabbit |
| Antibody Type | Recombinant monoclonal |
| Form | Liquid |
| Conjugate | Unconjugated |
| Storage | Shipped on blue ice; follow antibody-specific protocols for long-term storage |
| Quantity | 1 kit (4 antibodies, trial-size) |
| Intended Use | For research use only |
Scientific Context
- MMR Function – MSH2 pairs with MSH6 to form MutSα, while MLH1 pairs with PMS2 to form MutLα. Together, these complexes detect and repair mismatches in DNA, preventing the accumulation of mutations.
- Cancer Relevance – Defective MMR proteins cause microsatellite instability (MSI), a hallmark of Lynch syndrome and associated malignancies (colorectal, gastric, endometrial).
- Pathway Links – MMR proteins interact with DNA replication machinery, p53 signaling, and apoptosis pathways, making them critical biomarkers for cancer research.
The Abcam Mismatch Repair (MSH6, PMS2, MLH1, MSH2) Antibody Panel - Human (ab252190) is a powerful, validated toolkit for researchers investigating DNA repair mechanisms, genomic stability, and cancer biomarkers. With four high-performance recombinant rabbit monoclonal antibodies, this panel supports robust IHC and ICC/IF workflows for mismatch repair protein profiling in FFPE tissues and human cell samples.
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