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Bruker standard solvent, suitable for MALDI-MS, 50% ACN + 47.5% H2O + 2.5% TFA
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Specifications:
| Application | MALDI-MS | ||
| Storage Temperature | Ambient | ||
| Product Type | Standard Solutions | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Analytical grade | Formula | C₂HF₃O₂ |
The Bruker Standard Solvent is a high-quality liquid solution specifically formulated for use in MALDI-MS (Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry) applications. Its composition ensures optimal performance for sample ionization and analysis in MALDI-MS workflows. The solvent comprises 50% acetonitrile (ACN), 47.5% water (H2O), and 2.5% trifluoroacetic acid (TFA), providing excellent solubilization properties and compatibility with a wide range of biological and chemical analytes.
Key Specifications
| Property | Details |
|---|---|
| Linear Formula | CF₃COOH |
| CAS Number | 76-05-1 |
| Molecular Weight | 114.02 |
| Quality Level | 100 |
| Form | Liquid |
| Boiling Point (bp) | 72.4 °C (lit.) |
| Melting Point (mp) | -15 °C (lit.) |
| Density | 0.921 g/cm³ at 25 °C |
| Vapor Density | 3.9 (vs. air) |
| Vapor Pressure | 97.5 mmHg |
| Cation Traces | Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Zn (≤ 5-20 ppm) |
Composition
- Acetonitrile (ACN): 50%
A polar organic solvent that aids in analyte dissolution and matrix mixing. - Water (H₂O): 47.5%
Deionized water enhances ionization efficiency and sample homogeneity. - Trifluoroacetic Acid (TFA): 2.5%
A strong acid that reduces signal noise, improves ionization, and stabilizes analytes.
Applications and Use Cases
- MALDI-MS Analysis
- Used as a standard solvent for dissolving MALDI matrices and preparing analytes.
- Enhances matrix-assisted ionization of biomolecules such as peptides, proteins, and small organic molecules.
- Sample Preparation
- Compatible with hydrophobic and hydrophilic analytes, providing flexibility in sample preparation.
- Ideal for preparing co-crystals of matrix and analyte for mass spectrometry analysis.
- General Analytical Applications
- Useful in analytical chemistry for studies requiring high-purity solvents with controlled ionic content.
Quality and Safety Features
- Certified Quality: High-purity solvent with a quality level of 100.
- Low Trace Metals: Ensures minimal contamination with cation traces such as Fe, Na, and Zn at ≤20 ppm.
- Stable and Reliable: Vapor pressure and density are optimized for safe storage and consistent performance.
Chemical Identifiers
| Identifier | Details |
|---|---|
| SMILES String | OC(=O)C(F)(F)F |
| InChI | 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7) |
| InChI Key | DTQVDTLACAAQTR-UHFFFAOYSA-N |
Advantages
- Ready-to-use formulation optimized for MALDI-MS applications.
- Reduces background noise and enhances signal clarity in mass spectra.
- Supports reproducible and reliable experimental results.
This solvent is an essential tool for laboratories performing advanced proteomics, metabolomics, or chemical compound identification via MALDI-MS. Its stringent quality standards and precise formulation ensure superior results for research and diagnostic applications.