MALDI Biotyper® MBT Lipid Xtract™ Kit RUO

The MALDI Biotyper® MBT Lipid Xtract™ Kit RUO revolutionizes lipidomic analysis, enabling rapid detection of colistin resistance in Gram-negative bacteria by analyzing modifications in Lipid A. Leveraging the MALDI Biotyper® sirius system and negative ion mode, this kit offers an innovative, streamlined approach for resistance detection and microbial lipid analysis.

Key Features

1. Rapid Colistin Resistance Detection:
   • Identifies resistance based on distinctive Lipid A modifications, such as:
     • Phosphoethanolamine (pEtN)
     • 4-amino-4-deoxy-L-arabinose (L-Ara4N)
   • Detects chromosomal and plasmid-encoded resistance.
2. Negative Ion Mode Analysis:
   • Employs negative ion mode on the MALDI Biotyper® sirius for accurate lipid profiling.
   • Specialized for lipopolysaccharides (LPS) and Lipid A mass shifts.
3. Streamlined Sample Preparation:
   • Preparation completed in 15 minutes using the MBT Lipid Xtract™ Kit.
   • Simple workflow:
     • Bacterial lysis
     • Washing step
     • Mixing with Lipid Xtract matrix
   • Samples are applied to the MBT Biotarget 96 for MALDI-TOF analysis.
4. Dedicated Software:
   • The MBT HT LipidART software module automates spectra interpretation.
   • Provides clear insights into resistance mechanisms with just a few clicks.
5. High-Throughput Capability:
   • Results available in 30 minutes starting from bacterial colonies.
   • Enables rapid testing of multiple samples.

Applications

1. Colistin Resistance Detection:
   • Rapidly identifies last-resort antibiotic resistance in Gram-negative bacteria.
   • Crucial for combatting antimicrobial resistance (AMR) in healthcare settings.
2. Lipidomic Research:
   • Explores lipid modifications in microbial pathogens.
   • Supports studies on the role of Lipid A modifications in bacterial resistance.
3. Clinical and Public Health Microbiology:
   • Accelerates diagnostics in laboratories handling Gram-negative infections.
   • Supports infection control measures by identifying resistant strains.
4. Antimicrobial Stewardship Programs:
   • Guides treatment decisions by confirming colistin susceptibility.
   • Reduces reliance on broad-spectrum antibiotics.

Workflow

1. Sample Preparation:
   • Collect a small bacterial colony.
   • Perform lysis and wash steps with the MBT Lipid Xtract™ Kit.
   • Mix with Lipid Xtract matrix and apply to the MBT Biotarget 96.
2. Analysis:
   • Use the MALDI Biotyper® sirius system in negative ion mode.
   • Acquire lipid spectra in the lower mass range.
3. Interpretation:
   • Analyze spectra with the MBT HT LipidART software module.
   • Identify Lipid A modifications and interpret resistance mechanisms.
4. Report Generation:
   • Results are delivered in under 30 minutes, providing actionable insights.

Benefits

1. Speed and Efficiency:
   • Enables rapid detection of colistin resistance, critical for timely intervention.
   • Streamlined workflow minimizes sample preparation time.
2. Accuracy:
   • Negative ion mode enhances sensitivity and specificity for lipid analysis.
   • Identifies subtle mass shifts indicative of resistance mechanisms.
3. Ease of Use:
   • Simplifies lipidomic analysis with user-friendly software and protocols.
   • Reduces the technical expertise required for microbial lipid studies.
4. High-Throughput Testing:
   • Supports simultaneous analysis of multiple samples, improving lab productivity.
5. Cost-Effective Alternative:
   • Provides a practical solution for resistance detection compared to molecular methods.

Specific Use Cases

1. Healthcare Diagnostics:
   • Rapidly identify colistin-resistant Klebsiella pneumoniae or Escherichia coli in bloodstream infections.
2. AMR Surveillance Programs:
   • Monitor the prevalence of plasmid-encoded colistin resistance genes in hospital settings.
3. Lipidomics Research:
   • Study lipid modification pathways in Gram-negative bacteria and their role in resistance mechanisms.
4. Veterinary Microbiology:
   • Detect colistin resistance in zoonotic pathogens affecting livestock.

The MALDI Biotyper® MBT Lipid Xtract™ Kit RUO integrates lipidomics into microbiology workflows, providing a rapid, efficient, and accurate method for detecting colistin resistance. Its innovative use of negative ion mode, streamlined sample preparation, and dedicated software makes it an invaluable tool for combating antimicrobial resistance and advancing lipidomic research.