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Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Set
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• Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Set provides the complete collection of buffers and endotoxin-free reagents used in the PureLink Expi Endotoxin-Free plasmid purification kits, made available separately for laboratories requiring additional buffer capacity. This set ensures that users can extend the number of plasmid purifications or replace consumed reagents while maintaining the same level of quality and performance as the original kits.
Designed for flexibility, the buffer set supports up to 30 Maxi, 4 Mega, or 2 Giga preps and includes all reagents required for cell lysis, binding, washing, elution, and endotoxin removal. All buffers and solutions are supplied endotoxin-free, ensuring compatibility with advanced applications such as mammalian cell transfection, genome engineering, gene therapy, and plasmid vaccine research.
Key Features
- Complete Set – Includes all buffers and solutions required for PureLink Expi endotoxin-free plasmid purification workflows.
- Flexible Use – Supports up to 30 Maxi, 4 Mega, or 2 Giga plasmid preparations.
- Endotoxin-Free Certified – All buffers, elution water, and storage tubes are certified endotoxin-free.
- High Performance – Maintains the same purity and yield standards as included in PureLink Expi purification kits.
- Convenient Supply – Ideal for labs that run frequent plasmid preps or need to replenish depleted buffers.
Applications
- Large-scale purification of endotoxin-free plasmid DNA.
- Supplementary buffer supply for Expi Maxi, Mega, and Giga plasmid purification kits.
- Advanced workflows requiring DNA for mammalian transfection, sequencing, cloning, PCR, or in vitro transcription.
- Genome engineering, gene therapy, and vaccine research projects.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Buffer Set |
| Catalog Number | A33074 |
| Quantity | 1 set (sufficient for 30 Maxi, 4 Mega, or 2 Giga preps) |
| Application | Plasmid DNA purification (endotoxin-free) |
| Sample Type | Bacterial culture plasmid DNA |
| Prep Scale | Maxi, Mega, or Giga |
| Isolation Technology | Anion-exchange resin (buffer compatible) |
| Test Time | ~90 minutes (standard workflow) |
| Shipping Condition | Room temperature |
| Storage | Room temperature; RNase A at 4 °C |
| Unit Size | Each set |
Buffer Set Contents
- RNase A
- Resuspension Buffer (R3)
- Lysis Buffer (L7)
- Precipitation Buffer (N3)
- Equilibration Buffer (EQ1)
- Wash Buffer (W8)
- Elution Buffer (E4)
- TE Buffer (TE)
- Endotoxin Removal Buffer
- Lysis Indicator
- Endotoxin-free water
Unique Advantages
- Provides a cost-effective way to extend kit capacity without purchasing a full kit.
- Maintains endotoxin-free standards, ensuring compatibility with sensitive transfection applications.
- Reliable Invitrogen quality for consistent plasmid DNA prep performance.
The Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Set is an essential accessory for laboratories engaged in high-demand plasmid purification. By supplying all required buffers in endotoxin-free grade, this set ensures researchers can continue to achieve high yields of ultrapure plasmid DNA for critical applications such as transfection, genome editing, gene therapy, and vaccine development.