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Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit is a next-generation plasmid purification system optimized for producing ultra-pure, endotoxin-free DNA suitable for the most sensitive transfection applications. Using an enhanced anion-exchange membrane and a simplified protocol, the kit yields up to 1.5 mg plasmid DNA in just 90 minutes, eliminating the long processing times associated with traditional endotoxin-free plasmid purification methods.
Designed to remove endotoxins to levels below 0.1 EU/µg, this kit is ideal for workflows such as transfection of primary or sensitive cell lines, genome engineering, gene therapy research, and plasmid vaccine development. It also provides guaranteed compatibility with Gibco™ ExpiCHO™ and Expi293™ transient expression systems.
Key Features
- Ultra-Fast Protocol – Endotoxin-free plasmid DNA in ~90 minutes.
- High Yield – Isolate up to 1.5 mg of plasmid DNA per prep.
- Endotoxin-Free Output – Delivers DNA with <0.1 EU/µg endotoxin levels, ensuring suitability for sensitive cell lines.
- Superior Purity – Free from protein, RNA, and genomic DNA contamination.
- Expi System Compatible – Optimized for ExpiCHO™ and Expi293™ expression systems.
- Validated Endotoxin-Free Components – Includes certified endotoxin-free elution buffer, water, and plasmid storage tubes.
Applications
- Transfection of primary or sensitive mammalian cell lines.
- Genome engineering experiments (e.g., CRISPR-Cas9).
- Plasmid preparation for gene therapy and vaccine research.
- Cloning and ligation workflows.
- Sequencing and PCR.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Endotoxin-Free Maxi Plasmid Purification Kit |
| Catalog Numbers | A33073 (4 preps), A31217 (10 preps), A31231 (25 preps) |
| Isolation Technology | Enhanced anion-exchange membrane |
| Sample Type | E. coli bacterial culture |
| Prep Scale | Large-scale plasmid prep (>200 μg to >1 mg) |
| Yield | Up to 1.5 mg plasmid DNA |
| DNA Quality | <0.1 EU/µg endotoxin, A260/A280 > 1.8 |
| Test Time | ~90 minutes |
| Format | Spin/vacuum columns |
| Applications | Sequencing, cloning, transfection, PCR, genome engineering |
| Shipping Condition | Room temperature |
| Storage Condition | Store buffers at RT; RNase A at 4 °C |
| Unit Size | Each kit (4, 10, or 25 preps) |
Kit Contents
- Resuspension buffer
- Lysis indicator
- RNase A
- Lysis buffer
- Precipitation buffer
- Endotoxin removal buffer
- Wash buffer
- Elution buffer
- TE buffer
- Expi Maxi filtration column
- Expi Maxi DNA binding column
- 50 mL conical tubes
- Endotoxin-free water and plasmid storage tubes
Unique Advantages
- Produces ultra-clean, endotoxin-free plasmid DNA ready for demanding mammalian cell workflows.
- Faster and more efficient than traditional gravity-flow endotoxin-free plasmid preps.
- Endotoxin-free certified buffers and plastics eliminate contamination risk.
- Guaranteed performance with Expi expression systems for protein production.
The Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit sets a new benchmark for large-scale plasmid purification. Delivering up to 1.5 mg of endotoxin-free DNA in 90 minutes, it is ideal for transfections, genome engineering, gene therapy, and vaccine research. By combining speed, yield, and purity, it provides researchers with a robust and reliable solution for sensitive molecular biology applications.
- Pack Size: 4 Preps 10 Preps 25 Preps