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Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit, 2 Preps
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit is the ultimate solution for large-scale purification of ultra-pure plasmid DNA. Using an enhanced anion-exchange resin and a streamlined centrifugation- or vacuum-assisted protocol, the kit produces up to 15 mg plasmid DNA in just 2 hours, eliminating the long processing times of traditional endotoxin-free workflows.
DNA isolated with this kit meets the most demanding standards, with endotoxin levels <0.1 EU/µg, and ultralow contamination from protein, RNA, and genomic DNA. This makes it particularly suited for sensitive mammalian transfections, genome engineering, CRISPR workflows, and advanced gene therapy or plasmid vaccine development.
Key Features
- Exceptional Yield – Purify up to 15 mg plasmid DNA from large-scale E. coli cultures.
- Endotoxin-Free – <0.1 EU/µg, ensuring compatibility with the most sensitive applications.
- High Purity – Ultrapure DNA with A260/A280 > 1.8, free from protein, RNA, and genomic DNA.
- Fast Protocol – Complete plasmid purification in ~2 hours.
- Expi System Compatibility – Fully validated for use with Gibco™ ExpiCHO™ and Expi293™ transient expression systems.
- Certified Endotoxin-Free Components – Elution buffer, water, and storage tubes guarantee maximum purity.
Applications
- Large-scale plasmid preparation for mammalian cell transfection.
- Genome engineering and CRISPR applications.
- Gene therapy and plasmid vaccine development.
- High-throughput plasmid DNA prep for sequencing, cloning, PCR, and in vitro transcription.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Endotoxin-Free Giga Plasmid Purification Kit |
| Catalog Number | A31233 |
| Quantity | 2 preps |
| Isolation Technology | Enhanced anion-exchange resin |
| Sample Type | E. coli bacterial culture |
| Prep Scale | Giga-scale plasmid prep (~15 mg DNA) |
| Yield | Up to 15 mg plasmid DNA |
| DNA Quality | <0.1 EU/µg endotoxin, A260/A280 > 1.8 |
| Test Time | ~2 hours |
| Format | Spin/vacuum column |
| Applications | Sequencing, cloning, transfection, PCR, in vitro transcription |
| Shipping Condition | Room temperature |
| Storage Condition | Buffers at RT; RNase A at 4 °C |
| Unit Size | Each kit (2 preps) |
Kit Contents
- Resuspension Buffer
- Lysis Indicator
- RNase A
- Lysis Buffer
- Precipitation Buffer
- Endotoxin Removal Buffer
- Wash Buffer
- Elution Buffer
- TE Buffer
- Expi Giga Filtration Column
- Expi Giga DNA Binding Column
- Endotoxin-free elution buffer, water, and plasmid storage tubes
Unique Advantages
- Highest capacity in the PureLink Expi endotoxin-free portfolio, delivering up to 15 mg DNA per prep.
- Rapid purification workflow combining filtration and anion-exchange purification.
- Certified endotoxin-free buffers and consumables guarantee confidence in results.
- Tailored for advanced therapeutic research and highly sensitive molecular biology applications.
The Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit provides researchers with a scalable, ultra-fast, and endotoxin-free solution for producing the largest plasmid DNA yields in the PureLink range. Delivering up to 15 mg of transfection-grade DNA in only 2 hours, it is the optimal choice for applications in mammalian transfection, genome engineering, CRISPR, vaccine research, and gene therapy development.