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Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit, 4 Preps
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit is engineered for ultra-high-yield purification of transfection-grade plasmid DNA from E. coli cultures. Using a proprietary HiPure Expi anion-exchange resin and integrated vacuum filtration columns, the kit delivers rapid clarification and purification in a streamlined workflow, eliminating the need for centrifugation.
From 500 mL–1 L bacterial cultures, the kit yields 4–5 mg of ultrapure plasmid DNA in under 2 hours, with purity equivalent to two cesium chloride gradient passes. Endotoxin levels typically fall within 0.1–1 EU/µg, ensuring suitability for the most sensitive downstream applications such as mammalian cell transfection, genome engineering, and gene therapy research.
Key Features
- Exceptional Yield – Recover 4 mg plasmid DNA from 500 mL or >5 mg from 1 L bacterial culture.
- Transfection-Grade Purity – Low endotoxin levels (0.1–1 EU/µg) and A260/A280 > 1.8.
- Fast Protocol – Complete plasmid purification in ~90 minutes with vacuum assistance.
- Integrated Filtration – Vacuum-assisted columns allow lysate clarification without centrifugation.
- Safe & Efficient – No use of phenol, chloroform, CsCl, or ethidium bromide.
- High-Capacity Resin – Enhanced anion-exchange resin ensures rapid flow rate, high resolution, and efficient endotoxin removal.
Applications
- Transfection of mammalian and sensitive cell lines.
- Genome engineering and CRISPR workflows.
- Plasmid preparation for gene therapy and vaccine research.
- Large-scale plasmid prep for sequencing and cloning.
- PCR amplification and in vitro transcription.
- Restriction enzyme digestion and nucleic acid labeling.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Plasmid Megaprep Kit |
| Catalog Number | K210008XP |
| Quantity | 4 preps |
| Isolation Technology | HiPure Expi proprietary anion-exchange resin |
| Sample Type | E. coli bacterial culture |
| Sample Volume | 500 mL – 1 L |
| Prep Scale | Large-scale (>4 mg plasmid DNA) |
| Yield | ~4 mg from 500 mL; >5 mg from 1 L |
| DNA Quality | A260/A280 > 1.8 |
| Endotoxin Level | 0.1–1 EU/µg |
| Test Time | ~90 min – 2 hr |
| Format | Vacuum-assisted filter + anion-exchange column |
| Applications | Sequencing, cloning, transfection, PCR, in vitro transcription |
| Shipping Condition | Room temperature or wet ice |
| Storage | Store buffers and columns at room temperature |
| Unit Size | Each kit (4 preps) |
Kit Contents
- Equilibration Buffer
- Resuspension Buffer
- Lysis Buffer
- Precipitation Buffer
- RNase A
- Wash Buffer
- Elution Buffer
- TE Buffer
- Expi HiPure Mega Filtration Column
- Expi HiPure Mega DNA Binding Column
Unique Advantages
- Combines lysate filtration and DNA purification in one workflow.
- Yields the highest plasmid DNA amounts in the Invitrogen PureLink product line.
- Provides safe, rapid purification free of hazardous reagents.
- Delivers reproducible transfection-grade DNA suitable for advanced molecular biology.
The Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit is the ultimate solution for large-scale, endotoxin-free plasmid DNA purification, offering the highest yields in the PureLink™ range. With its proprietary resin, vacuum-assisted workflow, and guaranteed transfection-grade purity, it provides an efficient, safe, and reliable method for plasmid DNA production in sensitive applications such as transfection, genome engineering, gene therapy, and vaccine research.