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Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit, 4 Preps
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit is optimized for large-scale purification of ultra-pure, endotoxin-free plasmid DNA from E. coli cultures. Leveraging an enhanced anion-exchange membrane and a streamlined centrifugation- or vacuum-assisted protocol, the kit delivers up to 5 mg plasmid DNA in as little as 2 hours—making it one of the fastest and most efficient options for high-yield plasmid preparation.
DNA prepared with this kit meets the stringent purity standards required for advanced applications, with endotoxin levels <0.1 EU/µg, and minimal contamination from proteins, RNA, or genomic DNA. It is particularly suited for mammalian cell transfections, genome engineering, and in vivo gene therapy or vaccine research.
Key Features
- Ultra-High Yield – Purify up to 5 mg plasmid DNA from large-volume E. coli cultures.
- Endotoxin-Free – Delivers DNA with <0.1 EU/µg endotoxins, ideal for sensitive workflows.
- High Purity – DNA free from protein, RNA, and genomic DNA contaminants (A260/A280 > 1.8).
- Fast Workflow – Complete purification in ~2 hours, including precipitation.
- Expi System Compatible – Fully validated for use with Gibco™ ExpiCHO™ and Expi293™ expression systems.
- Endotoxin-Free Components – Includes certified endotoxin-free elution buffer, water, and plasmid storage tubes.
Applications
- Mammalian cell transfection (including primary and sensitive cell lines).
- Genome engineering workflows (e.g., CRISPR-Cas9).
- Gene therapy and plasmid vaccine research in vivo.
- Large-scale plasmid DNA prep for sequencing, cloning, PCR, and in vitro transcription.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Endotoxin-Free Plasmid Megaprep Kit |
| Catalog Number | A31232 |
| Quantity | 4 preps |
| Isolation Technology | Enhanced anion-exchange membrane |
| Sample Type | E. coli bacterial culture |
| Prep Scale | Large-scale plasmid prep (>5 mg DNA) |
| Yield | Up to 5 mg plasmid DNA |
| DNA Quality | <0.1 EU/µg endotoxin, A260/A280 > 1.8 |
| Test Time | ~2 hours |
| Format | Spin/vacuum columns |
| Applications | Transfection, cloning, sequencing, PCR, genome engineering |
| Shipping Condition | Room temperature |
| Storage Condition | Buffers at RT; RNase A at 4 °C |
| Unit Size | Each kit (4 preps) |
Kit Contents
- Resuspension Buffer
- Lysis Indicator
- RNase A
- Lysis Buffer
- Precipitation Buffer
- Endotoxin Removal Buffer
- Wash Buffer
- Elution Buffer
- TE Buffer
- Expi Mega Filtration Column
- Expi Mega DNA Binding Column
- Endotoxin-free elution buffer, water, and plasmid storage tubes
Unique Advantages
- Highest DNA yield in the PureLink™ Expi endotoxin-free range.
- Rapid protocol avoids gravity-flow columns, ensuring efficiency.
- Endotoxin-free certified buffers and plastics guarantee reliability.
- Provides DNA compatible with Expi expression systems and other high-sensitivity workflows.
The Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit offers unmatched capacity for endotoxin-free plasmid DNA isolation. Delivering up to 5 mg of ultrapure DNA in just 2 hours, it provides researchers with a robust, scalable solution for advanced applications including transfection of sensitive cells, genome engineering, plasmid vaccine research, and gene therapy development.