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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
High-purity restriction enzyme for precise DNA cleavage at GT^AC recognition sites
The Thermo Scientific™ RsaI restriction endonuclease specifically recognizes the sequence GT^AC and introduces a cut between the T and A nucleotides. Optimized to perform best at 37°C in 10X Buffer Tango, RsaI delivers reliable and reproducible results in a wide range of molecular biology applications.
Part of Thermo Scientific’s Five Buffer System, this enzyme is manufactured under stringent quality control, ensuring 100% activity under recommended conditions. For convenience in double digestions, the universal Tango buffer is included, and buffers are premixed with BSA to enhance stability and counteract contaminants.
Also available as a FastDigest™ enzyme for rapid DNA digestion.
Isoschizomers: AfaI, CviQI, RsaNI.
Key Features & Benefits
- Precise DNA Cleavage – Recognizes GT^AC and cleaves reproducibly at the specific site.
- Optimized Performance – Delivers maximum activity at 37°C in Tango buffer.
- Convenient Buffer System – Color-coded Five Buffer System with BSA premixed for stability.
- Double Digestion Ready – Compatible with universal Tango buffer for multi-enzyme reactions.
- High Quality Manufacturing – Stringent quality control for consistent, reproducible results.
- Broad Utility – Suitable for multiple DNA analysis and manipulation techniques.
Applications
- Molecular Cloning – DNA fragment generation for cloning workflows.
- Restriction Site Mapping – Determining DNA restriction patterns.
- Genotyping – Analyzing genetic variations.
- Southern Blotting – Preparing DNA samples for hybridization.
- Restriction Fragment Length Polymorphism (RFLP) – Genetic diversity studies.
- SNP Analysis – Detecting single nucleotide polymorphisms.
Recognition Sequence & Cleavage Pattern
| Strand | Sequence |
|---|---|
| 5′ | G T ↓ A C |
| 3′ | C A ↑ T G |
Technical Specifications
| Property | Details |
|---|---|
| Enzyme | RsaI |
| Recognition Site | GT^AC |
| Concentration | 10 U/μL |
| Unit Definition | One unit digests 1 μg of λ DNA in 1 hour under optimal conditions |
| Optimal Temperature | 37°C |
| Compatible Buffer | 10X Buffer Tango |
| Methylation Sensitivity | CpG Methylation-Sensitive; Not Dam or Dcm Methylation-Sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional Cloning |
| Storage | –20°C |
| Formulation | Supplied in recommended storage buffer with stabilizers |
Ordering Information
| Catalog Number | Quantity | Total Units |
|---|---|---|
| ER1121 | 1,000 units | 10 U/μL in 100 μL |
| ER1122 | 5,000 units | 10 U/μL in 500 μL |
The Thermo Scientific™ RsaI (10 U/μL) restriction enzyme provides precision, consistency, and versatility for molecular cloning and DNA analysis workflows. With high activity in optimal conditions, compatibility with double digestion protocols, and a robust buffer system, RsaI is a dependable choice for researchers requiring accurate restriction site cleavage in a variety of genetic applications.
- Pack Size: 1,000 units 5,000 units