Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase, Colorless

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an advanced polymerase designed to enhance the efficiency, specificity, and sensitivity of PCR reactions. Available in both colorless and green formats, it employs antibody-based inhibition to prevent non-specific amplification and ensures high-quality results in a variety of applications.

Key Features

Enhanced PCR Performance

• Higher Specificity and Sensitivity:
  • Antibody-based hot-start mechanism ensures DNA polymerase activity is inhibited at room temperature, reducing non-specific amplification.
• Better Yield:
  • Achieves high yields of target amplicons, even from low-template DNA samples.
• Wider Amplicon Range:
  • Capable of amplifying DNA fragments up to 6 kb in routine applications.

Optimized Workflow

• Convenient Setup:
  • Reactions can be set up at room temperature without affecting specificity or yield.
• Pre-Optimized Buffer:
  • Supplied with a 10X DreamTaq Buffer containing magnesium chloride, minimizing the need for reaction optimization.

Green Format Options:

• DreamTaq Green Buffer:
  • Contains a density reagent and two tracking dyes for direct loading of PCR products onto agarose gels.
• Colorless Format:
  • Ideal for applications requiring downstream fluorescence or absorbance detection.

Applications

• Routine PCR
• Genotyping
• Colony PCR
• Reverse Transcription PCR (RT-PCR)
• Generation of PCR Products for TA Cloning

Specifications

Product Availability

Advantages

1. High Productivity:
   • Achieve reliable and reproducible results with minimal reaction setup effort.
2. Versatility:
   • Suitable for a wide range of PCR applications, from routine to more complex setups.
3. Time-Saving:
   • Direct gel loading with DreamTaq Green Buffer simplifies workflows.
4. Stability:
   • Robust enzyme performance across a variety of annealing temperatures and Mg²⁺ concentrations.

Safety and Handling

• Storage Conditions: Store at -5 °C to -30 °C for long-term stability.
• Shipping Conditions: Shipped on dry ice to maintain enzyme activity.

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase offers the ideal combination of specificity, sensitivity, and convenience for researchers requiring high-quality PCR results in diverse applications.

Comparison: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase vs. OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Key Differences

1. Ease of Use:
   • DreamTaq™: Requires manual addition of components, offering flexibility for customization.
   • OneTaq® Quick-Load®: Ready-to-use master mix simplifies setup and minimizes error.
2. Performance with GC-Rich Templates:
   • DreamTaq™: Moderate performance for GC-rich templates.
   • OneTaq® Quick-Load®: Designed for better performance with difficult templates.
3. Amplicon Size Range:
   • DreamTaq™: Up to 6 kb.
   • OneTaq® Quick-Load®: Up to 10 kb for genomic DNA and up to 40 kb for lambda DNA.
4. Direct Gel Loading:
   • Both products offer this feature but through different buffer formulations.
5. Applications:
   • DreamTaq™: Focuses on basic PCR needs, TA cloning, and genotyping.
   • OneTaq® Quick-Load®: Broader application range, including challenging templates.

Choose Thermo Scientific™ DreamTaq™ for basic PCR workflows requiring flexibility and OneTaq® Quick-Load® for streamlined workflows or challenging templates.