Liofilchem™ MTS™ Ampicillin-sulbactam (2:1) AMS 0.016-256, MIC Test strips

Liofilchem™ MTS™ Ampicillin-Sulbactam (2:1) AMS MIC Test Strips provide an innovative solution for precise quantitative determination of the minimum inhibitory concentration (MIC) of Ampicillin-Sulbactam, a beta-lactam and beta-lactamase inhibitor combination, against a wide range of bacterial pathogens. The strips are designed for use in antimicrobial susceptibility testing (AST), enabling healthcare professionals and researchers to assess bacterial resistance and guide therapeutic decisions accurately.

Key Features

• Dual Antibiotic Synergy Testing: The test strip is impregnated with a predefined gradient of Ampicillin and Sulbactam in a fixed 2:1 ratio, allowing reliable detection of the combined activity.
• Wide MIC Range: Provides a gradient range of 0.016 to 256 µg/mL for comprehensive susceptibility profiling.
• Accurate MIC Determination: Enables direct and precise measurement of MIC values, critical for assessing bacterial resistance.
• Innovative Gradient Technology: Uses a continuous concentration gradient embedded in a thin, biodegradable cellulose strip for optimal diffusion on agar plates.
• Compliant with Guidelines: Conforms to international standards, including CLSI and EUCAST, for antimicrobial susceptibility testing.
• Ease of Use: Pre-calibrated and ready-to-use strips simplify workflow in clinical, research, and pharmaceutical laboratories.

Intended Use

• To determine the MIC of Ampicillin-Sulbactam for Gram-positive and Gram-negative bacterial isolates.
• Particularly useful for testing beta-lactamase-producing bacteria, including Enterobacteriaceae, Pseudomonas spp., Staphylococcus spp., and Acinetobacter spp..
• Applicable in hospital microbiology laboratories, research facilities, and surveillance programs monitoring antimicrobial resistance.

Test Procedure

1. Preparation:
   • Prepare Mueller-Hinton Agar (or suitable medium for fastidious organisms) following CLSI or EUCAST guidelines.
   • Allow the medium to solidify and ensure its surface is dry before inoculation.
2. Inoculation:
   • Prepare a bacterial suspension equivalent to 0.5 McFarland standard.
   • Use a sterile swab to evenly streak the suspension across the agar plate.
3. Application:
   • Using sterile forceps, place an MTS™ Ampicillin-Sulbactam AMS strip onto the inoculated agar surface with the printed MIC scale facing upward.
4. Incubation:
   • Incubate the plate at 35 ± 2°C in an appropriate atmosphere (aerobic or with 5% CO₂ as needed) for 16–20 hours.
5. Interpretation:
   • After incubation, observe the inhibition ellipse. The MIC is the value where the bacterial growth intersects the strip.
   • Interpret the results following CLSI or EUCAST breakpoints.

Product Specifications

• Gradient Range: 0.016–256 µg/mL.
• Antibiotic Ratio: Ampicillin to Sulbactam in a fixed 2:1 ratio.
• Material: Biodegradable cellulose strip.
• Sterility: Sterile, individually packed.
• Compatibility: Suitable for a wide variety of bacterial pathogens.

Quality Control

Each batch of Liofilchem™ MTS™ Ampicillin-Sulbactam AMS is tested using QC strains to ensure performance:

Storage and Stability

• Store at 2–8°C in original packaging, protected from light and moisture.
• Use before the expiration date printed on the package.
• Avoid freezing and excessive exposure to heat or humidity.

Ordering Information

Applications

• Clinical microbiology laboratories for patient care.
• Research settings for studying bacterial resistance mechanisms.
• Pharmaceutical industries for evaluating the efficacy of Ampicillin-Sulbactam.
• Epidemiological studies to monitor resistance trends.

Precautions

• For in vitro diagnostic use only.
• Handle all materials and specimens as potentially infectious.
• Dispose of used materials following local biohazard regulations.

Liofilchem™ MTS™ Ampicillin-Sulbactam AMS Test Strips deliver precision and ease of use, ensuring reliable susceptibility testing for improved therapeutic outcomes.