Liofilchem™ EnteroPluri-Test System for Enterobacteriaceae Identification

Liofilchem® EnteroPluri-Test

The Liofilchem® EnteroPluri-Test is an advanced 12-sector diagnostic system designed for the identification of Enterobacteriaceae and other Gram-negative, oxidase-negative bacteria. This system enables the simultaneous inoculation of multiple media, allowing the observation of 15 biochemical reactions for microbial identification.

Key Features

1. Comprehensive Diagnostic Capability:
   • 12-Sector System: Includes specialized culture media for detecting key biochemical reactions.
   • 15 Biochemical Reactions: Evaluates enzymatic activity, carbohydrate fermentation, and other metabolic pathways.
2. Rapid and Reliable:
   • Incubation Time: Provides results within 18–24 hours at 36 ± 1°C.
   • Colorimetric Results: Identifies microorganisms based on color changes in culture media.
3. Streamlined Identification Process:
   • Results are analyzed using a code number derived from the interpretation of biochemical reactions.
   • EnteroPluri-Test Code Book: Facilitates the identification of Enterobacteriaceae and other Gram-negative bacteria.
4. Ease of Use:
   • Simultaneous inoculation of all media ensures consistent and efficient testing.
   • Compact design reduces the need for multiple individual tests.
5. Flexible Packaging Options:
   • Available in packs of 10 tests (Code: 78618) or 25 tests (Code: 78619).

Applications

1. Clinical Microbiology:
   • Ideal for diagnosing infections caused by Enterobacteriaceae and other oxidase-negative, Gram-negative bacteria.
2. Industrial Microbiology:
   • Useful in food, water, and environmental testing to monitor contamination by Enterobacteriaceae.
3. Research Laboratories:
   • Supports research in bacterial phenotyping and metabolic studies.

Biochemical Reactions Evaluated

The EnteroPluri-Test analyzes the following reactions to identify microorganisms:

• Glucose Fermentation
• Gas Production
• Lysine Decarboxylation
• Ornithine Decarboxylation
• H2S Production
• Indole Production
• Adonitol Fermentation
• Lactose Fermentation
• Arabinose Fermentation
• Sorbitol Fermentation
• Voges-Proskauer Test
• Dulcitol Fermentation
• Phenylalanine Deamination
• Urease Activity
• Citrate Utilization

Procedure

1. Inoculation:
   • Inoculate the system with a bacterial suspension using the integrated wire loop.
2. Incubation:
   • Incubate at 36 ± 1°C for 18–24 hours.
3. Result Interpretation:
   • Observe and record color changes in each sector.
   • Generate a biochemical reaction code using the EnteroPluri-Test Code Book for bacterial identification.

Additional Reagents and Accessories

Packaging Information

Benefits

Why Choose EnteroPluri-Test?

• Efficiency: Simplifies bacterial identification with a single, compact system.
• Speed: Provides rapid results, reducing diagnostic turnaround time.
• Reliability: Trusted Liofilchem® quality ensures accurate and reproducible results.
• Versatility: Applicable in clinical, industrial, and research laboratories.

The Liofilchem® EnteroPluri-Test is a versatile and efficient solution for identifying Enterobacteriaceae and related Gram-negative bacteria, enabling laboratories to deliver faster and more accurate results.

Result Interpretation for Liofilchem® EnteroPluri-Test

The results of the Liofilchem® EnteroPluri-Test are interpreted by evaluating the color changes in each of the 12 sectors, which correspond to specific biochemical reactions. Each color change indicates a positive or negative result for the respective reaction. A unique biochemical profile code is then generated, which is used to identify the microorganism.

Step-by-Step Interpretation Process

1. Observation of Color Changes:
   • After 18–24 hours of incubation at 36 ± 1°C, observe the 12 sectors of the EnteroPluri-Test.
   • Compare the colors in each sector with the provided reference chart.
2. Record Positive and Negative Reactions:
   • Assign a value of positive (+) for color changes indicating a specific reaction and negative (-) for no color change.
   • For example:
     • Yellow in the Glucose Fermentation sector indicates acid production (positive reaction).
     • Red in the Indole Production sector after adding Kovac's reagent indicates a positive indole reaction.
3. Biochemical Reaction Profile:
   • Divide the test results into five groups of reactions based on the sectors.
   • Assign a numerical value to each positive reaction within a group. The numerical values are typically weighted (e.g., 1, 2, 4) and added to generate a group code.
4. Generate a Biochemical Profile Code:
   • Combine the group codes in order to create a five-digit profile code representing the biochemical reactions of the microorganism.
5. Identify the Microorganism:
   • Use the EnteroPluri-Test Code Book to match the biochemical profile code with the corresponding microorganism.
   • The code book contains an extensive list of Enterobacteriaceae and other Gram-negative bacteria with their respective codes.

Example of Interpretation

Biochemical Profile Code:

• Positive reactions in a group are assigned numerical values (e.g., 4, 2, 1).
• For instance, if a group has reactions 4 (positive), 2 (positive), and 1 (negative), the sum is 6 for that group.
• Combining the results from all five groups generates the final profile code.

Example Code: 65231

Microorganism Identified: Refer to the EnteroPluri-Test Code Book to match the code with the bacterial species.

Additional Notes

• Positive and Negative Controls: Perform tests with known positive and negative control organisms to validate results.
• Reagent Addition: For certain tests (e.g., indole and Voges-Proskauer), reagents must be added post-incubation to confirm results.

Comparison between Liofilchem™ EnteroPluri-Test System and API 20E

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