Promega 100bp DNA Ladder, 250μl

The Promega 100bp DNA Ladder is a pre-mixed DNA size marker used for gel electrophoresis. This ladder is designed to assist in the size determination of DNA fragments by providing a series of 11 fragments ranging from 100bp to 1,000bp in 100bp increments, along with a band at 1,500bp. It is ideal for use in agarose gel electrophoresis and provides clear size comparison for DNA samples, making it a valuable tool in DNA analysis.

The 100bp DNA Ladder is supplied with 6X Blue/Orange Loading Dye, which allows for easy tracking of the DNA migration during electrophoresis. It is provided at a concentration of 0.13µg/µl and is ready for use, ensuring reliable and consistent results across experiments.

Key Features & Benefits:

1. Wide Range of Fragments:
   • The ladder contains 11 DNA fragments with sizes from 100bp to 1,000bp, allowing for accurate size determination of PCR products or other DNA fragments within this range.
   • Additionally, it includes a 1,500bp band, making it ideal for more flexible sizing applications.
2. Easy-to-Use:
   • Pre-mixed with 6X Blue/Orange Loading Dye, which simplifies sample loading and monitoring of electrophoresis. This helps you visualize migration in real-time and track the DNA’s movement through the gel.
   • Ready for immediate use without additional preparation.
3. High Sensitivity:
   • Supplied at a concentration of 0.13µg/μl, providing optimal signal intensity for use in agarose gel electrophoresis, even with low DNA quantities.
4. Ambient Temperature Shipping:
   • The ladder ships at ambient temperature, making it easy to store and transport without the need for special handling or refrigeration.
5. Convenient Loading:
   • The recommended loading amount is 5μl/lane, making it convenient to use across standard gel formats and typical applications.

Applications:

• DNA Fragment Size Determination: Ideal for determining the size of PCR products, digested DNA, or cloned fragments in agarose gel electrophoresis.
• Molecular Biology: Used in gene expression analysis, cloning, and genetic fingerprinting for accurate sizing of DNA fragments.
• PCR Analysis: Suitable for confirming the size of PCR amplicons and verifying DNA fragment integrity.
• Cloning and Sequencing: Useful for sequence analysis and confirming the success of cloning experiments.

Specifications:

Storage:

• Store at ambient temperature. No refrigeration required.

The Promega 100bp DNA Ladder is a convenient, reliable, and high-performance tool for DNA fragment size determination. It offers clear resolution with a wide range of fragments and is pre-mixed with loading dye for ease of use. Whether you're analyzing PCR products, gene clones, or digested DNA, this ladder ensures accurate, reproducible results every time.