Thermo Scientific™ TopVision™ Low Melting Point Agarose

The TopVision Low Melting Point Agarose is a high-quality agarose gel designed for sensitive applications in molecular biology, particularly where low-melting-point properties are essential. Its superior performance, low DNA/RNA binding, and excellent gel transparency make it a reliable choice for analytical and preparative nucleic acid electrophoresis.

Key Features

1. Low Melting Point:
   • Melting point of 65.5°C allows for gentle handling of nucleic acids during in-gel enzymatic processing and nucleic acid recovery.
2. High Purity:
   • Certified DNase- and RNase-free, ensuring contamination-free results.
3. GQ (Genetic Quality) Certification:
   • Guarantees that recovered nucleic acids are suitable for downstream applications, including enzymatic reactions like restriction digestion and ligation.
4. Versatile Buffer Compatibility:
   • Performs optimally at concentrations of 0.7–2% in all standard electrophoresis buffer systems.
5. Low Nucleic Acid Binding:
   • Ensures high recovery efficiency of DNA and RNA from gels after electrophoresis.
6. Excellent Gel Transparency:
   • Enables clear visualization of bands, improving the accuracy of nucleic acid analysis.

Applications

1. Analytical Electrophoresis:
   • Separation and analysis of nucleic acids for molecular biology research.
2. Preparative Electrophoresis:
   • Recovery of nucleic acids from gels for downstream processes.
3. In-Gel Enzymatic Processing:
   • Enzymatic reactions, such as restriction digestion and ligation, directly within the gel.
4. Routine Molecular Biology Techniques:
   • Preparation of DNA gels for cloning, sequencing, and other applications.
5. RNA Analysis:
   • Suitable for sensitive RNA-based experiments.

Technical Specifications

Why Choose TopVision Low Melting Point Agarose?

• Reliability: GQ certification ensures that recovered nucleic acids are functional for critical downstream applications.
• Ease of Use: Low melting point facilitates efficient nucleic acid recovery and enzymatic processing.
• Versatility: Suitable for a broad range of molecular biology workflows, from routine analysis to advanced in-gel processing.
• High Purity: DNase- and RNase-free, ensuring sample integrity in sensitive applications.

The TopVision Low Melting Point Agarose is an essential tool for researchers requiring precise and efficient nucleic acid analysis and recovery in molecular biology workflows.