Invitrogen™ UltraPure™ Low Melting Point Agarose

The Invitrogen™ UltraPure™ Low Melting Point Agarose is a high-quality polysaccharide used for the size-based separation of nucleic acids in gel electrophoresis. Designed for routine analysis and recovery of DNA and RNA, this agarose offers strong gel integrity, efficient nucleic acid recovery, and versatile applications. It is especially suitable for in-gel manipulations and other specialized experiments.

Key Features

1. Low Melting and Gelling Points:
   • Melting Point: 65.5°C, below the denaturation temperature of nucleic acids.
   • Fluidity: Remains fluid at 37°C, ideal for enzymatic manipulations.
   • Setting Temperature: Rapidly sets below 25°C, forming a firm gel.
2. Strong Gel Structure:
   • Enhanced gel strength for reduced breakage and easier handling.
3. Versatility:
   • Suitable for various applications, including:
     • DNA/RNA analysis and recovery.
     • In-gel enzymatic processes.
     • Tissue and cell culture.
     • Viral plaque assays.
     • Protein electrophoresis (e.g., Ouchterlony and radial immunodiffusion assays).
4. Improved Packaging:
   • Environmentally friendly packaging with 75% less plastic.
   • Easy-pour spout minimizes spills and contamination risks.
5. High Purity:
   • Free of detectable DNase and RNase, ensuring reliable nucleic acid recovery.
   • Performance tested for parameters like gel strength, gelling/melting temperatures, sulfate content, and electroendosmosis.

Applications

1. Nucleic Acid Gel Electrophoresis:
   • Ideal for size separation of DNA and RNA fragments (100–30,000 bp).
2. Nucleic Acid Recovery:
   • Facilitates the extraction of DNA/RNA for downstream applications, such as cloning or sequencing.
3. Protein Electrophoresis:
   • Applicable in antigen-antibody interaction assays (e.g., Ouchterlony) and antigen quantitation assays (e.g., radial immunodiffusion).
4. In-Gel Enzymatic Reactions:
   • Enzyme manipulations like restriction digestion and ligation.
5. Cell and Tissue Culture:
   • Useful in embedding tissues or cells for various culture experiments.
6. Viral Plaque Assays:
   • Suitable for virology applications requiring plaque formation analysis.

Technical Specifications

Key Benefits

• Reliability: High gel integrity ensures consistent performance in various applications.
• Versatility: Suitable for both nucleic acid and protein analysis, as well as specialized assays.
• Eco-Friendly Packaging: Supports sustainable laboratory practices with reduced waste.
• Purity: DNase- and RNase-free for contamination-free results.
• Ease of Handling: Easy-pour spout and strong gel structure simplify laboratory workflows.

Why Choose UltraPure™ Low Melting Point Agarose?

• Optimized for a wide range of molecular biology applications, including DNA and RNA analysis, recovery, and enzymatic processing.
• Reliable performance backed by rigorous quality testing.
• Environmentally conscious packaging that aligns with sustainable lab goals.

This agarose is an excellent choice for researchers requiring high-quality, versatile gel media for electrophoresis and related applications.