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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Syphilis ELISA is an indirect immunoenzyme assay designed for the qualitative detection of antibodies against Treponema pallidum in human serum or plasma. This test is intended for the laboratory diagnosis of syphilis and can be used in both routine and automated ELISA systems.
The assay employs Treponema pallidum antigens adsorbed onto the surface of polystyrene wells, enabling the detection of specific IgG and/or IgM antibodies. Results are visualized through a colorimetric reaction using TMB substrate, ensuring high sensitivity, specificity, and reproducibility.
Available Kits
| Product | Reference | Nº Tests | Class |
|---|---|---|---|
| Syphilis ELISA IgG | G1060 | 96 | CE |
| Syphilis ELISA IgG + IgM | T1060 | 96 | CE |
Key Features
- Comprehensive antibody detection – available formats for IgG or combined IgG + IgM detection.
- Direct serum dilution & treatment – sample preparation directly in the well with optional sorbent treatment.
- High assay reliability – samples and controls are equally processed to minimize pipetting variability.
- User-friendly workflow – colour-coded plates with individual break-apart wells for flexible use.
- Stable reagents – lyophilized conjugates when required, ensuring long-term stability.
- Ready-to-use convenience – coloured, liquid reagents prepared for immediate use.
- Automation compatible – suitable for automated ELISA systems in medium-to-high throughput labs.
Principle of the Test
- Antibodies present in the patient serum or plasma bind to Treponema pallidum antigens coated on the polystyrene surface.
- Unbound immunoglobulins are washed away.
- An enzyme-labelled anti-human globulin is added, binding to the antigen-antibody complex.
- After washing, TMB substrate develops a blue colour, which turns yellow upon addition of the acid stop solution.
- The resulting optical density is proportional to the concentration of antibodies against Treponema pallidum.
Applications
- Laboratory diagnosis of syphilis.
- Screening and confirmation testing in clinical microbiology laboratories.
- Epidemiological surveillance of Treponema pallidum infections.
- Suitable for manual or automated ELISA platforms.
The Syphilis ELISA kits (IgG and IgG+IgM) provide sensitive, reliable, and convenient solutions for the detection of syphilis antibodies. With CE certification, high stability, and compatibility with automated platforms, these assays are ideal for routine clinical diagnostics, public health laboratories, and syphilis surveillance programs.
- Detectable Antibody: IgG IgG + IgM