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Thermo Scientific™ Tween 20 Washing Buffer, 1% in PBS (10X)
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Concentrated PBS-T Buffer for Protein Solubilization, Immunohistochemistry, and Immunoblotting Applications
Specifications:
| Application | Protein Assays & Analysis, Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Tween 20 Washing Buffer, 1% in PBS (10X) is a high-quality concentrated phosphate-buffered saline detergent solution designed for a wide range of molecular biology and immunodetection workflows. This PBS-T formulation contains 1% Tween 20, a gentle nonionic surfactant widely used to improve washing efficiency, reduce nonspecific antibody binding, and support membrane protein solubilization.
Supplied as a viscous liquid concentrate, this buffer is ideal for routine laboratory applications including Western blotting, immunohistochemistry (IHC), ELISA washing steps, and membrane protein preparation. Its 10X stock format provides convenience, reproducibility, and reduced preparation time.
Key Features and Benefits
Nonionic Surfactant for Effective Washing and Blocking
Tween 20 helps prevent nonspecific antibody binding and can function as a blocking agent in immunoblotting assays, improving assay sensitivity and reducing background.
Useful for Membrane Protein Solubilization
Tween 20 is commonly applied in membrane protein isolation workflows, supporting the solubilization of membrane-associated proteins.
Compatible with Immunohistochemistry Applications
Frequently added to IHC buffers to enhance reagent penetration and reduce nonspecific staining.
Supports Pre-Extraction of Membranes
Used for removing peripheral proteins during membrane preextraction steps, improving downstream protein analysis.
Convenient Concentrated Stock Solution
Supplied as a 10X concentrate, allowing rapid dilution to working strength for daily laboratory use.
Research Use Only
Intended strictly for research laboratory applications.
Applications
Thermo Scientific™ PBS-T (1% Tween 20, 10X) is suitable for:
- Western blot membrane washing
- Immunoblotting blocking and wash buffer preparation
- ELISA assay wash steps
- Immunohistochemistry buffer supplementation
- Solubilization of membrane proteins
- Membrane preextraction to remove peripheral proteins
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | Tween 20 Washing Buffer |
| Composition | 1% Tween 20 in PBS |
| Concentration | 10X Stock Solution |
| Physical Form | Viscous liquid |
| Chemical Type | Buffer |
| Recommended Storage | Keep cold |
| Intended Use | Research Use Only (RUO) |
Ordering Information
| Catalog Number | Pack Size | Alternate Name |
|---|---|---|
| J63314.AP | 500 mL | J63314-AP |
| J63314.K2 | 1 L | J63314-K2 |
| J63314.K3 | 2 L | J63314-K3 |
Thermo Scientific™ Tween 20 Washing Buffer, 1% in PBS (10X), is a versatile and effective PBS-T concentrate for laboratories performing immunoblotting, immunohistochemistry, ELISA, and membrane protein workflows. With its optimized surfactant formulation, it enhances washing efficiency, reduces nonspecific antibody binding, and supports reproducible results in demanding molecular biology applications. The convenient concentrated format and cold storage recommendation make it a reliable choice for research laboratories.
- Pack Size: 1L 500 mL 2L