Thermo Scientific™ Blocker™ FL Fluorescent Blocking Buffer (10X), 100 mL

Enhanced signal clarity in fluorescent western blotting applications

The Thermo Scientific™ Blocker™ FL Fluorescent Blocking Buffer (10X) is a ready-to-dilute blocking solution designed to minimize nonspecific binding and reduce background fluorescence in western blotting applications. Specifically optimized for fluorescent detection methods, this blocking buffer increases the signal-to-noise ratio, ensuring clearer, more accurate protein visualization on nitrocellulose and PVDF membranes.

Blocker FL is compatible with most near-infrared and visible fluorescent probes, helping to maintain low background while maximizing the detection sensitivity of primary and secondary antibodies in multiplex or single-channel imaging applications.

Key Features

• Reduces Background Fluorescence: Minimizes nonspecific binding in fluorescent western blots
• Optimized for Fluorescent Detection: Enhances signal clarity and sensitivity
• Broad Compatibility: Works with both nitrocellulose and PVDF membranes
• Flexible Dilution: Supplied as a 10X concentrate for user-defined working strength
• Ready-to-Use Format: Easy preparation—just dilute 1 part buffer with 9 parts ultrapure water
• Reliable Performance: Maintains low background in multiplex and NIR fluorescence systems

Applications

• Western blotting using fluorescent-labeled antibodies
• Multiplex blot detection with near-infrared or visible dyes
• Fluorescent protein detection in low-expression systems
• Fluorescence-based imaging of nitrocellulose or PVDF membranes

Specifications

The Thermo Scientific™ Blocker™ FL Fluorescent Blocking Buffer is an essential reagent for researchers conducting fluorescent western blotting who require low background, high sensitivity, and reliable reproducibility. Its optimized formulation ensures superior blocking performance across a variety of fluorescent detection platforms, making it ideal for high-precision protein analysis.