Thermo Scientific™ Restore™ Western Blot Stripping Buffer

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Specifications:
Application Western Blot
Storage Temperature 2-8°C
Product Type Buffer Forms Liquid
Product Brand Thermo Fisher Scientific™
Product Grade Molecular Biology

Restore™ Western Blot Stripping Buffer is a gentle yet highly effective solution designed to remove primary and secondary antibodies from Western blot membranes, allowing multiple rounds of probing without the need to re-run SDS-PAGE gels. Compatible with nitrocellulose and PVDF membranes, Restore buffer enables researchers to conserve precious samples, optimize antibody concentrations, and validate multiple targets from a single blot.

Unlike homemade or harsh stripping buffers, Restore buffer is formulated to preserve immobilized antigens, ensuring reliable reprobing and clean backgrounds during subsequent immunodetection. It is odor-free, easy to use, and ideal for chemiluminescent Western blot workflows.

Key Features and Benefits

  • Efficient antibody removal – strips both primary and secondary antibodies without harming target proteins
  • Preserves sample integrity – gentle formulation maintains antigen structure and membrane integrity
  • Mercaptan-free – odorless solution for safer and more pleasant use
  • Cost-saving – eliminates the need to prepare duplicate gels and blots
  • Rapid protocol – stripping complete in 5 to 15 minutes
  • Compatible with chemiluminescence – optimized for use with HRP substrates such as SuperSignal™

Applications

  • Strip and reprobe Western blot membranes for detection of multiple targets
  • Adjust antibody concentrations to optimize signal without repeating gel runs
  • Cost-effective solution for working with rare, limited, or high-value samples

Protocol Summary

  1. Wash blot to remove any residual chemiluminescent substrate.
  2. Incubate in Restore Stripping Buffer for 5–15 minutes at 37 °C (room temperature may suffice for low-affinity antibodies).
  3. Wash thoroughly in TBS-T or PBS-T.
  4. Test removal of prior antibodies (optional).
  5. Reblock and reprobe with new antibody.

Ordering Information

Catalog NumberVolumeFormatEstimated Blots (Mini-Gels)
2106230 mLBottle~5–10 blots
21059500 mLBottle~100–150 blots
210635 LBottle~1000–1500 blots

Storage Conditions

  • Store at room temperature or 4 °C
  • Stable for up to 12 months
  • Ready to use—no dilution or mixing required


Thermo Scientific™ Restore™ Western Blot Stripping Buffer provides a reliable, efficient, and non-destructive solution for reusing Western blot membranes in multi-target protein detection workflows. Whether you're validating antibody specificity, conserving limited sample, or optimizing signal quality, Restore buffer supports flexibility, reproducibility, and cost-efficiency in your blotting experiments.

  • Pack Size:   500 mL 30 mL Bag-in-box (Cubitainer), 5L

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