Thermo Scientific™ Pierce™ PVDF Transfer Membranes, 0.2 μm

The Pierce™ PVDF Transfer Membranes are high-quality polyvinylidene difluoride (PVDF) membranes designed for superior protein and nucleic acid transfer in Western, Southern, and Northern blotting applications. These membranes offer exceptional binding capacity, mechanical strength, and compatibility with various detection methods, making them a reliable choice for sensitive and high-precision experiments.

Key Features

1. High Binding Capacity:
   • Optimized for low-abundance protein detection.
   • Binding capacities for:
     • Goat IgG: 448 μg/cm²
     • BSA: 340 μg/cm²
     • Insulin: 262 μg/cm²
2. Small Pore Size (0.2 μm):
   • Ideal for transferring small molecular weight proteins and peptides.
   • Suitable for amino acid analysis and working with as little as 10 pmoles of protein.
3. Mechanical Strength and Reusability:
   • Strong and durable, allowing for stripping and re-probing in repeated experiments.
   • Compatible with various organic solvents, acids, and mild bases.
4. Detection Compatibility:
   • Works seamlessly with multiple detection techniques:
     • Staining
     • Chemiluminescence
     • Radiolabeling
     • For fluorescent western blotting, choose the Low-Fluorescence PVDF Transfer Membrane.
5. Durability and Stability:
   • Resistant to most organic solvents, making it versatile for various transfer conditions.
6. Flexible Formats:
   • Available as:
     • Rolls for customizable use.
     • Pre-cut membranes for convenience.
7. Ease of Use:
   • Recommended to pre-activate the membrane with 100% alcohol before use for optimal protein binding and transfer.

Specifications

Applications and Use Cases

1. Western Blotting

• Purpose:
  • Efficient transfer and detection of proteins for antibody-based experiments.
• Use Case:
  • Detect low-abundance proteins in cell lysates or tissue extracts using chemiluminescence or fluorescence.

2. Southern Blotting

• Purpose:
  • Transfer and detection of DNA fragments.
• Use Case:
  • Analyze genomic DNA after digestion and gel electrophoresis.

3. Northern Blotting

• Purpose:
  • Transfer and detection of RNA molecules.
• Use Case:
  • Study RNA expression patterns in different cell types or under various conditions.

4. Small Protein and Peptide Transfer

• Purpose:
  • Optimize detection of low molecular weight proteins and peptides.
• Use Case:
  • Use for precise peptide analysis and low-concentration protein samples in proteomics.

5. Protein Binding and Reprobing

• Purpose:
  • Bind proteins and strip/reprobe membranes for repeated analyses.
• Use Case:
  • Perform sequential probing of the same blot for different targets, reducing experimental costs.

6. Amino Acid Analysis

• Purpose:
  • Analyze amino acid sequences in proteins.
• Use Case:
  • Use the high-binding membrane to capture and study amino acids in protein digests.

Storage and Handling

1. Storage Instructions:
   • Store flat at ambient temperature.
   • Keep away from chemical vapors to avoid damage to the pore structure.
2. Handling Precautions:
   • Pre-activate the membrane with 100% alcohol before transfer to ensure high protein binding.
   • Avoid prolonged exposure to solvents that might disrupt membrane integrity.

Advantages

1. Exceptional Sensitivity:
   • Enables detection of proteins and peptides at low concentrations.
2. Versatile Applications:
   • Compatible with multiple blotting and detection techniques.
3. Reusability:
   • Strong mechanical durability allows for stripping and re-probing.
4. Customizable and Convenient:
   • Available in rolls for flexibility or pre-cut formats for convenience.
5. Durability:
   • Resistant to organic solvents, acids, and mild bases, ensuring robustness under various conditions.

Recommended Usage Protocol

1. Membrane Preparation:
   • Pre-activate the membrane with 100% alcohol for a few seconds and rinse with water before use.
2. Gel Transfer:
   • Perform transfer using standard wet or semi-dry transfer protocols.
3. Blocking:
   • Block the membrane with an appropriate blocking buffer to minimize non-specific binding.
4. Detection:
   • Use your preferred detection method (e.g., staining, chemiluminescence, fluorescence).
5. Stripping and Re-probing:
   • Strip the membrane gently and reprobe for additional protein targets if necessary.

Why Choose Pierce™ PVDF Transfer Membranes?

1. Superior Binding Capacity:
   • High protein and nucleic acid binding for sensitive detection.
2. Durable and Reusable:
   • Withstands stripping and re-probing, saving resources and time.
3. Versatile Formats:
   • Flexible roll and pre-cut options for diverse experimental needs.
4. Robust for Various Techniques:
   • Compatible with chemiluminescence, fluorescence, and other detection methods.
5. High Sensitivity for Small Proteins:
   • 0.2 μm pore size ensures optimal results for low molecular weight proteins and peptides.

The Pierce™ PVDF Transfer Membranes provide an excellent solution for researchers seeking high sensitivity, versatility, and durability in protein and nucleic acid transfer applications.