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Thermo Scientific™ Pierce™ Protein G Plus Agarose
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Specifications:
| Application | Immunohistochemistry, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Protein G Plus Agarose is a premium, high-capacity affinity resin designed for robust purification of immunoglobulin G (IgG) from various mammalian species. This advanced resin features recombinant Protein G covalently immobilized at high density onto crosslinked 6% beaded agarose (CL-6B), delivering superior binding performance and consistency across multiple purification cycles.
Engineered for low nonspecific binding and high specificity, Protein G Plus Agarose is especially suited for purifying mouse, human, goat, cow, and sheep IgGs, including challenging isotypes like human IgG3 and mouse IgG1.
Key Features & Benefits
- High Binding Capacity: Binds >20 mg human IgG/mL of resin for efficient processing of small to moderate sample volumes.
- Broad Isotype Compatibility: Captures IgGs from a wide range of species, particularly effective for mouse IgG1 and human IgG3.
- Recombinant Protein G: Lacks albumin and cell-surface binding domains, minimizing nonspecific interactions.
- Stability and Reusability: Immobilized using AminoLink™ chemistry for stable, leach-resistant covalent coupling, ensuring consistent yields and resin longevity.
- Versatile Compatibility: Suitable for column chromatography, spin-column formats, or gravity-flow applications.
- Efficient Elution and Recovery: Retains protein function and integrity post-purification with elution across mild to standard conditions.
Applications
- Affinity purification of polyclonal and monoclonal IgGs from serum, ascites, or culture supernatant.
- Immunoprecipitation workflows requiring low background and high specificity.
- Downstream antibody labeling, analysis, or assay development.
Technical Specifications
| Property | Details |
|---|---|
| Product Code | 22851 (2 mL), 22852 (10 mL) |
| Binding Capacity | >20 mg human IgG/mL resin |
| Resin Base | CL-6B crosslinked 6% agarose |
| Ligand | Recombinant Protein G |
| Particle Size | 45–165 μm |
| Support pH Stability | pH 2–14 (short term); pH 3–13 (long term) |
| Exclusion Limit | 10,000 to 4,000,000 Da |
| Flow Rate | ~1 mL/min (1 cm column) |
| Max Pressure | <25 psi (1.5 bar) |
| Format | Liquid suspension in 20% ethanol |
| Storage Temperature | 4°C |
| Unit Size | Each (bottle format) |
The Pierce™ Protein G Plus Agarose provides enhanced performance in antibody purification, offering a high-capacity, species-compatible, and reusable solution for affinity-based workflows. Whether used for routine IgG enrichment or complex immunological studies, this resin is a dependable tool for achieving high-purity results with minimal loss or contamination.
- Pack Size: 10 mL 2 mL