Thermo Scientific™ Pierce™ Protein G Agarose

Thermo Scientific™ Pierce™ Protein G Agarose is a premium-quality affinity chromatography resin designed for efficient purification of immunoglobulin G (IgG) antibodies from a variety of mammalian species. Utilizing recombinant Protein G covalently immobilized onto crosslinked 6% beaded agarose (CL-6B), this resin provides excellent performance in terms of binding capacity, specificity, and stability across repeated purification cycles.

With a binding capacity of 11–15 mg human IgG per mL of resin, this product is ideal for small-scale affinity purification protocols requiring high purity and recovery of antibodies. The resin is optimized for both manual and automated applications, offering compatibility with microcentrifuge spin columns or gravity flow columns.

Key Features & Benefits

• Broad Species Compatibility: Effectively purifies IgG antibodies from mouse (including IgG1), human, goat, cow, and sheep. Exhibits enhanced binding to human IgG3 and rat IgG subclasses.
• High Binding Efficiency: Provides robust recovery with minimal nonspecific binding, even in complex biological samples.
• Recombinant Protein G: Lacks albumin-binding sites to minimize background contamination and nonspecific interactions.
• Stable Coupling Chemistry: Immobilized using AminoLink™ chemistry for superior leach resistance and reusability across multiple cycles.
• pH Versatility: Supports a wide pH stability range (pH 2–14 short-term; 3–13 long-term), facilitating various buffer systems.
• Ready-to-Use Format: Supplied as a slurry with storage buffer; compatible with most common antibody purification protocols.

Technical Specifications

Applications

• Purification of polyclonal or monoclonal IgG antibodies from serum, ascites, or hybridoma culture supernatants
• Immunoprecipitation workflows
• Antibody-based assay development
• Sample preparation for downstream analysis such as ELISA, Western blot, or mass spectrometry

The Pierce™ Protein G Agarose is a trusted solution for researchers and protein chemists requiring reliable, high-performance antibody purification. Its broad IgG subtype compatibility and durable immobilization chemistry make it especially valuable in laboratories working with a range of species or isotypes, including challenging subclasses like human IgG3 and mouse IgG1. Whether in R&D or diagnostic assay development, this resin ensures consistent, high-yield antibody purification with minimal background.