Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO)

The Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) is a highly selective and chromogenic medium designed for the isolation, enumeration, and presumptive identification of Listeria spp. and Listeria monocytogenes in food and environmental samples. Following broth enrichment, this medium facilitates the rapid and reliable differentiation of Listeria species through chromogenic and enzymatic activity.

Listeria species produce blue-green colonies by cleaving the chromogen X-glucoside through ß-glucosidase activity. Listeria monocytogenes and Listeria ivanovii are further identified by their ability to produce lecithinase, which hydrolyzes lecithin in the medium, creating an opaque, white halo around colonies.

Key Features

1. Selective and Chromogenic:
   • Differentiates Listeria spp. and L. monocytogenes through chromogenic activity and lecithinase production.
2. ISO-Compliant:
   • Conforms to ISO 11290-1:2017 and ISO 11290-2:2017 standards for the detection and enumeration of Listeria spp.
3. Inhibitory Agents:
   • Contains lithium chloride, polymyxin B, nalidixic acid, and amphotericin, suppressing the growth of competing bacteria, yeasts, and molds.
4. Rapid Results:
   • Clearly differentiated colonies of Listeria spp. within 22 hours when following validated methods.
5. Broad Application:
   • Suitable for food safety testing, environmental hygiene monitoring, and regulatory compliance.

Applications

1. Food Microbiology

• Detection and enumeration of Listeria spp. and L. monocytogenes in food samples, including:
  • Dairy products
  • Meat and poultry
  • Seafood
  • Processed and ready-to-eat foods

2. Environmental Monitoring

• Monitoring Listeria contamination in food production facilities, equipment, and environmental swabs.

3. Regulatory and Compliance Testing

• Conforms to ISO standards, ensuring compliance with international food safety and environmental hygiene requirements.

Specifications

Preparation Instructions

1. Suspend: Suspend 71.6g of Brilliance Listeria Agar (ISO) in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation to dissolve completely. Do not autoclave.
3. Supplement: Add Brilliance Listeria Agar (ISO) Selective Supplements (SR0257 and SR0258) aseptically.
4. Dispense: Pour the prepared medium into sterile Petri dishes and allow it to solidify.
5. Inoculate: Following broth enrichment, inoculate the medium and incubate as required.

Advantages

1. Highly Selective and Reliable: Suppresses non-target organisms, ensuring accurate detection of Listeria spp.
2. Rapid and Easy-to-Read Results: Differentiates Listeria spp. (blue-green colonies) and L. monocytogenes (white halo) in under 24 hours.
3. Regulatory Compliance: Meets ISO 11290 standards for global food safety testing.
4. Comprehensive Workflow: Integrates seamlessly with Listeria Precis™ Detection and Enumeration Method.
5. Broad Sample Compatibility: Effective for diverse food and environmental matrices.

Diagnostic Features

Colony Appearance

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Brilliance™ Listeria Agar (ISO)
• Catalog Numbers:
  • CM1212B (500g)
  • CM1212T (5kg)
• Yield: 7L per 500g, 70L per 5kg

The Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) is an advanced chromogenic medium that simplifies the detection, enumeration, and presumptive identification of Listeria spp. and L. monocytogenes. With its rapid differentiation, robust selectivity, and ISO compliance, it is an indispensable tool for microbiological testing in food safety, environmental monitoring, and regulatory compliance workflows.