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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ DyLight™ 488 NHS Ester is a high-intensity, amine-reactive fluorescent dye designed for the efficient labeling of antibodies, proteins, and other primary amine-containing biomolecules. With excitation and emission maxima at 493/518 nm, this dye offers brightness, photostability, and water solubility superior to traditional dyes such as FITC, Cy2™, and Alexa Fluor™ 488.
The NHS ester-activated format ensures specific, stable conjugation to lysine residues and N-terminal amino groups via amide bond formation, making it ideal for immunofluorescence microscopy, IHC, ELISA, and other fluorescence-based detection methods.
Key Features and Benefits
High Fluorescence and Photostability
- Brighter and more photostable than FITC, Cy2™, and LI-COR™ dyes.
- Performs reliably across a broad pH range (pH 4–9).
Specific NHS Ester Chemistry
- Reacts with primary amines (-NH₂) on lysine side chains or N-termini to form stable amide bonds.
- Suitable for labeling IgG, enzymes, and recombinant proteins.
High Solubility
- Excellent aqueous solubility enables high dye-to-protein ratios without precipitation.
- Dye can be dissolved in anhydrous DMF for flexible labeling workflows.
Flexible Use Formats
- Available as individual dye aliquots or as part of complete antibody labeling kits.
- Supported by standard and microscale labeling protocols for different sample volumes.
Applications
-
Primary antibody labeling for:
- Immunofluorescence microscopy
- Immunohistochemistry (IHC)
- Western blotting
- ELISA
-
Protein and enzyme labeling for:
- In vitro fluorescence assays
- In vivo imaging (compatible platforms)
- Fluorescence-based binding or localization studies
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | 46402 (1 mg), 46403 (5 × 50 μg) |
| Reactive Moiety | NHS ester (N-hydroxysuccinimide) |
| Target Functional Group | Primary amines (-NH₂) |
| Label Type | DyLight™ 488 |
| Excitation / Emission | 493 nm / 518 nm |
| Solubility | DMF (Dimethylformamide) |
| Formulation | Lyophilized solid |
| Storage Conditions | Store desiccated at –20°C, protected from light |
| Shipping Condition | Ambient temperature |
| Unit Size | 1 mg or 5 × 50 μg |
Related Products
- DyLight™ 488 Antibody Labeling Kit
- DyLight™ 488 Microscale Antibody Labeling Kit
DyLight™ 488 NHS Ester is a superior choice for researchers requiring bright, photostable, and efficient fluorescent labeling of proteins or antibodies. With enhanced solubility, specific amine-reactivity, and compatibility with standard fluorescence protocols, it is ideal for applications ranging from cellular imaging to antibody-based detection assays. When paired with the appropriate labeling kit, it streamlines the workflow for producing high-quality fluorescent conjugates.
- Pack Size: 1 mg 5 x 50 μg