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Thermo Scientific™ DyLight™ Antibody Labelling Kit
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Specifications:
| Application | Western Blot, Immunofluorescence, Fluorescence-Based Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ DyLight™ Antibody Labeling Kits provide a complete solution for covalently labeling antibodies or proteins with amine-reactive DyLight™ fluorophores across the visible and near-infrared spectrum. Each kit includes all necessary reagents to perform three separate labeling reactions using 0.5–1 mg of antibody or similar protein and to purify labeled conjugates for direct use in fluorescence-based detection applications.
DyLight dyes are known for their superior brightness, high photostability, broad pH stability (pH 4–9), and excellent aqueous solubility—making them ideal for use in imaging, immunoassays, and in vitro/in vivo protein tracking.
Key Features and Benefits
High Performance Across the Spectrum
- DyLight dyes deliver strong fluorescence signals and excellent photostability.
- Available in 10 colors from UV to near-IR (350–800 nm emission range).
- Compatible with standard filter sets and detection platforms.
Fast, Convenient Labeling Workflow
- Fluorescently labeled antibodies are ready in ~90 minutes with minimal hands-on time.
- Kits include NHS-activated DyLight dyes, borate buffer, spin columns, and purification resin.
Specific NHS-Ester Chemistry
- Labels primary amines on lysine residues or N-terminal groups.
- Forms stable amide bonds—no reversible linkage.
Multiple Formats Available
- Standard Kits for 3 × 1 mg labeling reactions
- Microscale Kits for 5 × 100 μg labeling reactions
- Single-use dye vials minimize reagent waste and ensure consistency
Applications
- Immunofluorescence microscopy (IF)
- Immunohistochemistry (IHC)
- Western blot detection
- ELISA-based fluorescence detection
- Flow cytometry and high-content screening
- In vitro or in vivo molecular imaging
Technical Specifications
| Property | Details |
|---|---|
| Labeling Target | Antibodies, Proteins |
| Reactive Group | NHS Ester (Amine-reactive) |
| Label Type | DyLight™ Fluor Dyes |
| Excitation / Emission | Depends on catalog number (see table below) |
| Labeling Scale | 0.5 to 1 mg per reaction (standard kit) |
| Formulation | Lyophilized dye, buffer, resin, columns |
| Storage Conditions | Store at –20°C, protected from light |
| Shipping Condition | Ambient temperature |
| Kit Contents | 5 × 15 µg DyLight NHS Ester, Borate Buffer (1 mL), Purification Resin (5 mL), 5 Spin Columns, 10 Collection Tubes |
| Unit Size | 3 reactions |
Available DyLight™ Antibody Labeling Kits
| Catalog Number | Excitation / Emission (nm) | Fluorophore |
|---|---|---|
| 53024 | 490 / 525 | DyLight 488 |
| 53020 | 400 / 420 | DyLight 405 |
| 53044 | 590 / 617 | DyLight 594 |
| 53046 | 638 / 658 | DyLight 633 |
| 53056 | 679 / 702 | DyLight 680 |
| 53062 | 784 / 814 | DyLight 800 |
| 62275 | 346 / 442 | DyLight 350 |
| 84530 | 562 / 576 | DyLight 550 |
| 84535 | 652 / 672 | DyLight 650 |
| 84538 | 749 / 775 | DyLight 755 |
Thermo Scientific™ DyLight™ Antibody Labeling Kits provide a streamlined, flexible platform for creating bright, stable, and specific fluorescent antibody conjugates. Whether for high-resolution microscopy, flow cytometry, or high-throughput analysis, these kits simplify the preparation of fluorescent probes tailored to your application and instrument requirements.
- Fluorophore: DyLight™ 488 DyLight™ 405 DyLight™ 594 DyLight™ 633 DyLight™ 680 DyLight™ 800 DyLight™ 350 DyLight™ 550 DyLight™ 650 DyLight™ 755