SolisFAST® 1-step RT-PCR Kit with UNG

The SolisFAST® 1-step RT-PCR Kit with UNG offers a fast, sensitive, and highly specific solution for analyzing RNA targets. This kit combines reverse transcription and PCR amplification in a single tube, reducing handling time, minimizing variation, and streamlining workflows. Optimized for speed, SolisFAST® delivers end-point RT-PCR results in under 1 hour, with the ability to detect as little as 0.1 pg of RNA and support multiplexing of up to 5 RNA targets simultaneously.

Incorporating Salini UNG® (Uracil-N-Glycosylase) and dUTPs, the kit prevents carryover contamination, while AmpliBoost™ RT-PCR enhancer ensures high sensitivity and reliable amplification of GC-rich templates. The proprietary Stability TAG™ technology guarantees enzyme stability, allowing convenient room-temperature handling and robust performance.

Key Features & Benefits

• Fast Workflow – RT-PCR results in less than 1 hour.
• High Sensitivity – Detects as little as 0.1 pg of RNA.
• Multiplexing Capability – Amplify up to 5 targets per reaction.
• Carryover Contamination Prevention – Preblended UNG enzyme with dUTPs.
• AmpliBoost™ Enhancer – Improves sensitivity and amplification of GC-rich/complex RNA templates.
• Stable & Reliable – Proprietary Stability TAG™ technology ensures robustness and room-temp stability.
• Convenient Formats – Ready-to-load version available for direct gel electrophoresis.

Applications

• Gene expression analysis
• Detection of RNA presence/absence
• Virus detection
• Cloning of RNA templates (up to 2 kb)
• Singleplex and multiplex RT-PCR assays

Kit Components

• 4× One-step HOT SolisFAST® Mix with UNG – Contains HOT SolisFAST® DNA polymerase, Salini UNG®, optimized buffer, dNTPs with dUTP, MgCl₂
• 40× One-step RT Mix – Includes SOLIScript® and FIREScript® reverse transcriptases plus RiboGrip® RNase inhibitor
• 5× AmpliBoost™ RT-PCR Enhancer – Improves amplification of GC-rich and complex templates
• Nuclease-free water

Product Specifications

Ordering Information

The SolisFAST® 1-step RT-PCR Kit with UNG delivers rapid, sensitive, and contamination-resistant RNA analysis with superior performance for both routine and challenging workflows. By combining reverse transcription and PCR into a single reaction, incorporating UNG contamination prevention, and offering enhanced GC-rich template amplification with AmpliBoost™, this kit is ideal for gene expression studies, viral detection, cloning, and multiplex assays in research laboratories.