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Specifications:
| Application | qPCR, PCR, Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes, Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Solis Biodyne | ||
| Product Grade | Molecular Biology | ||
The RiboGrip® RNase Inhibitor is a novel, in silico–engineered, protein-based RNase inhibitor designed to provide robust protection of RNA samples from degradation. Incorporating Solis BioDyne’s patented Stability TAG™ technology, RiboGrip® is exceptionally heat-stable, retaining full activity for up to 1 hour at 60°C, and allowing room temperature shipping and handling.
This advanced inhibitor effectively inactivates RNase A, RNase B, and RNase C through non-covalent, non-competitive binding. Its high-concentration formulation (220 U/µl) and lyo-compatible, glycerol-free option make it particularly suited for lyophilization workflows.
Key Features & Benefits
- Exceptional Stability – Fully active after 1 hour at 60°C; suitable for assays requiring high incubation temperatures.
- Novel Engineering – In silico–designed protein with unique stabilization through Stability TAG™.
- Effective RNA Protection – Efficiently protects RNA samples even at low DTT concentrations.
- High Concentration – Supplied at 220 U/µl for flexible use in various workflows.
- Lyo-Compatible – Glycerol-free formulation available for lyophilization to beads or cakes.
- Wide Assay Compatibility – Suitable for RT-(q)PCR, RT-LAMP, RNA-Seq, IVT, and cDNA synthesis.
- Protocol Compatibility – Included in FIREScript®, SOLIScript®, and Solis BioDyne one-step RT-PCR kits; also compatible with Illumina single-cell sequencing protocols.
Applications
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Preventing RNA degradation during:
- First-strand cDNA synthesis
- RT-PCR and RT-qPCR
- RT-LAMP isothermal amplification
- RNA isolation and purification
- In vitro transcription and translation
- RNA sequencing workflows
Product Specifications
| Attribute | Detail |
|---|---|
| Concentration | 220 U/µl |
| Specificity | RNase A, RNase B, RNase C |
| Origin | Recombinant mammalian chimeric protein |
| Source | Purified from E. coli carrying RiboGrip® gene |
| Molecular Weight | 47 kDa (monomer) |
| Unit Definition | One unit inhibits activity of 5 ng RNase A by 50% |
| Recommended Use | 0.5–2.5 U/µl final concentration |
| Storage Buffer | 50% glycerol, 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1 mM EDTA, 5 mM DTT, stabilizers |
| Storage | –20°C (routine); stable at RT for 1 month |
| Shipping | Room temperature |
Ordering Information
| Catalog Number | Size | Reaction Capacity |
|---|---|---|
| 06-26-2000U | 2000 U | 100 rxn |
| 06-26-4000U | 4000 U | 200 rxn |
| 06-26-010kU | 10,000 U | 500 rxn |
| 06-26-050kU | 50,000 U | 5000 rxn |
The RiboGrip® RNase Inhibitor (220 U/µl) provides next-generation RNA protection with unmatched stability and flexibility. With Stability TAG™ technology, lyo-compatible formulations, and proven performance across RNA workflows, RiboGrip® is the reliable choice for safeguarding RNA in research, sequencing, and diagnostic assay development.
- Pack Size: 2000 U, Sufficient for 100 Rxn 4000 U, Sufficient for 200 Rxn 10,000 U, Sufficient for 500 Rxn 100,000 U, Sufficient for 5000 Rxn