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Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium with Gamborg’s Vitamins (MS + B5), Powder
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Specifications:
| Application | Plant Cell Culture, Agricultural and horticultural research | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Plant Cell Culture | ||
The Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium with Gamborg’s Vitamins is an advanced plant tissue culture medium that combines the macro- and micronutrients of the classic Murashige and Skoog (1962) formulation with the vitamin profile described by Gamborg et al. (1968). This modified formulation provides enhanced nutritional support for a wide range of plant culture systems.
Supplied as a basal medium without sucrose or agar, it offers flexibility for researchers to customize formulations by adding carbon sources, gelling agents, and plant growth regulators, making it suitable for both routine and specialized plant biotechnology applications.
Key Features
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MS Basal Salts with Gamborg (B5) Vitamins
Combines essential nutrients with an enriched vitamin composition for improved growth response. -
Customizable Medium Formulation
Designed for supplementation with sucrose, agar, auxins, and cytokinins. -
High Quality (Quality Level 200)
Ensures consistency and reproducibility in plant tissue culture workflows. -
Broad Application Versatility
Suitable for diverse plant species and experimental systems. -
Powder Format for Flexible Preparation
Allows precise control of medium composition.
Applications
- Seed germination and seedling development studies
- Plant transformation and genetic research
- Suspension culture systems (e.g., Nicotiana plumbaginifolia)
- Root culture studies (e.g., Artemisia artemisiifolia)
- General plant tissue culture and micropropagation
- Agricultural and plant biotechnology research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Murashige and Skoog Basal Medium with Gamborg’s Vitamins |
| Synonyms | MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media |
| Brand | Sigma-Aldrich™ |
| SKU | M0404 |
| Form | Powder |
| Composition | MS macro- & micronutrients + Gamborg (B5) vitamins |
| Preparation | 4.4 g per liter |
| Quality Level | 200 |
| Technique | Plant cell culture |
| Applications | Agriculture, plant biotechnology |
| Storage Temperature | 2–8 °C |
| Shipping Conditions | Ambient |
| MDL Number | MFCD00240976 |
Packaging & Ordering Information
| SKU | Pack Size | Description |
|---|---|---|
| M0404-1L | 1 L | Powder for preparation of 1 liter medium |
| M0404-10L | 10 L | Powder for preparation of 10 liters medium |
Preparation Guidelines (General)
- Dissolve 4.4 g of powder per liter of distilled water
- Add sucrose (typically 20–30 g/L) as a carbon source
- Add agar or alternative gelling agents if required
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Supplement with plant growth regulators such as:
- Auxins (e.g., IAA)
- Cytokinins (e.g., Kinetin)
- Adjust pH (typically 5.6 – 5.8)
- Sterilize by autoclaving
The Sigma-Aldrich™ Murashige and Skoog Basal Medium with Gamborg’s Vitamins offers a flexible and nutritionally enhanced foundation for plant tissue culture applications. Its combination of MS basal salts and B5 vitamin supplementation supports consistent growth and development, making it suitable for a wide range of plant biotechnology, transformation, and propagation workflows.
- Pack Size: Powder for preparation of 10 liters medium Powder for preparation of 1 liter medium