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Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium with Sucrose and Agar
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Specifications:
| Application | Plant Cell Culture, Agricultural and horticultural research | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Plant Cell Culture | ||
The Sigma-Aldrich™ Murashige and Skoog (MS) Basal Medium with Sucrose and Agar is a complete, ready-to-use plant tissue culture medium formulated according to the original Murashige and Skoog (1962) composition. This formulation includes macro- and micronutrients, vitamins, sucrose (carbon source), and agar (gelling agent), providing a convenient, all-in-one solution for plant culture applications.
Designed for consistency and ease of preparation, this medium is suitable for a wide range of plant species and is widely used in plant biotechnology, agricultural research, and micropropagation laboratories.
Key Features
-
Complete MS Formulation
Contains basal salts, vitamins, sucrose, and agar in a single powder. -
Ready-to-Prepare Medium
Eliminates the need for separate addition of carbon source and gelling agent. -
Optimized for Plant Tissue Culture
Supports cell division, differentiation, and regeneration. -
High Quality (Quality Level 200)
Ensures reliable and reproducible performance. -
Broad Species Compatibility
Suitable for diverse plant types including model and crop species.
Applications
- General plant tissue culture and micropropagation
- Seed germination and seedling development
- Callus induction and organogenesis
- Genetic transformation and transgenic plant studies
- Agricultural and horticultural research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Murashige and Skoog Basal Medium |
| Synonym | MS Basal Medium |
| Brand | Sigma-Aldrich™ |
| SKU | M9274 |
| Form | Powder |
| Composition | MS salts, vitamins, sucrose, agar |
| Preparation | 42.4 g per liter |
| Quality Level | 200 |
| Technique | Plant cell culture |
| Applications | Agriculture, plant biotechnology |
| Storage Temperature | 2–8 °C |
| Shipping Conditions | Ambient |
| MDL Number | MFCD00240976 |
Packaging & Ordering Information
| SKU | Pack Size | Description |
|---|---|---|
| M9274-1L | 1 L | Powder for preparation of 1 liter medium |
| M9274-10L | 10 L | Powder for preparation of 10 liters medium |
Preparation Guidelines (General)
- Dissolve 42.4 g of powder per liter of distilled water
- Heat to dissolve agar completely
- Adjust pH (typically 5.6 – 5.8)
- Sterilize by autoclaving
Application Examples
- Culturing Arabidopsis transgenic seeds for cotyledon development
- Callus induction in pearl millet (Pennisetum glaucum)
- Tissue culture of Rosmarinus officinalis (rosemary)
The Sigma-Aldrich™ Murashige and Skoog Basal Medium with sucrose and agar provides a convenient and reliable all-in-one solution for plant tissue culture applications. Its complete formulation simplifies preparation while ensuring consistent performance across a wide range of plant research and propagation workflows.
- Pack Size: Powder for preparation of 10 liters medium Powder for preparation of 1 liter medium