Salini UNG® Uracil-N-Glycosylase

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Specifications:

Application	PCR
Storage Temperature	-20°C
Product Type	Enzymes and Substrates	Forms	Liquid
Product Brand	Solis Biodyne
Product Grade	Molecular Biology

Overview
Resource

Salini UNG® Uracil-N-Glycosylase is a fast, robust, and heat-labile enzyme designed to prevent PCR carryover contamination by efficiently removing uracil from DNA. Derived from the Salinivibrio genus, this unique enzyme features Solis BioDyne’s patented Stability TAG technology, which ensures exceptional room temperature stability and reliable performance without the need for cold-chain logistics.

Its heat-labile nature ensures no reactivation after heat inactivation, making it highly suitable for sensitive downstream applications such as Sanger sequencing. With a 30-second reaction time, tolerance to inhibitors, and glycerol-free formulation availability, Salini UNG® provides speed, convenience, and reliability in molecular workflows.

Key Features

Fast and Efficient – 30-second reaction time for rapid uracil removal.
Heat-Labile – Compatible with downstream Sanger sequencing; no reactivation after heat inactivation.
Exceptional Stability – Stable for 3 months at 25 °C and 2 weeks at 37 °C.
Stability TAG Technology – Proprietary modification for superior room-temperature stability.
Carryover Prevention – Eliminates uracil-containing DNA to prevent false positives in PCR and LAMP assays.
Versatile Applications – Supports cloning, SNP detection, DNA repair studies, and mutagenesis.
Convenient Handling – Stable shipping and setup at room temperature; glycerol-free formulation available.

Applications

PCR and LAMP carryover contamination prevention
Enhancing cloning efficiency of PCR products
Site-directed mutagenesis
Protein–DNA interaction studies
Glycosylase-mediated single nucleotide polymorphism detection (GMPD)
DNA repair and mutation detection research
SNP genotyping

Technical Specifications

Property	Details
Concentration	1 U/µL
Source	Purified from E. coli overproducing plasmid with Salini UNG® gene
Working Temperature	25–40 °C
Inactivation	5 min at 70 °C
Working Concentration (qPCR)	0.025–0.04 U/µL
Working Concentration (PCR)	0.005–0.01 U/µL
Stability at 25 °C	≥ 3 months
Stability at 37 °C	≥ 2 weeks
Storage Buffer	50% glycerol, 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers
Routine Storage	–20 °C
Shipping	Ambient temperature

Ordering Information

Product	Catalog Number	Pack Size
Salini™ UNG Uracil-N-Glycosylase	31-01-0000S	25 U
Salini™ UNG Uracil-N-Glycosylase	31-01-00100	100 U
Salini™ UNG Uracil-N-Glycosylase	31-01-00100-5	500 U
Salini™ UNG Uracil-N-Glycosylase	31-01-00100-10	1000 U
Salini™ UNG Uracil-N-Glycosylase	31-01-00100-20	2000 U
Salini™ UNG Uracil-N-Glycosylase	31-01-00100-200	20,000 U

The Salini UNG® Uracil-N-Glycosylase is a high-performance, heat-labile glycosylase optimized for PCR contamination prevention, molecular biology workflows, and SNP detection. Its unmatched room-temperature stability, rapid activity, and compatibility with Sanger sequencing make it an essential reagent for researchers requiring speed, reliability, and contamination control in nucleic acid amplification applications.