Nunc™ Immulon™ 96 Well plates, Non-sterile, F-Bottom
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Well Volume: 300 μL
Specifications:
| Application | Cell culture, Cell Analysis |
| Storage Temperature | Room Temperature |
| Product Type | Plate |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Clear Round-Bottom Immuno Nonsterile 96-Well Plates are designed for reliable and reproducible solid-phase immunoassays. These plates are available with a variety of surface treatments optimized for hydrophilic or hydrophobic binding, making them ideal for both quantitative and qualitative assays, including ELISA and antibody sandwich assays.
Key Features:
- Multiple Surface Options for Optimal Binding:
- MaxiSorp (Hydrophilic): Ideal for antibody sandwich assays and glycoprotein binding.
- PolySorp (Hydrophobic): Best suited for adsorbing hydrophobic molecules.
- Immulon 1B (Hydrophobic): Suitable for a wide range of hydrophobic molecules.
- Immulon 2HB (Slightly Hydrophilic): Binds a diverse range of biomolecules.
- Clear Polystyrene Construction:
- Ensures consistent optical properties for colorimetric assays.
- Round-Bottom Well Design:
- Facilitates uniform reagent distribution and efficient mixing during assays.
- Alphanumeric Labeling:
- Simplifies sample identification and tracking.
- Non-Sterile Format:
- Suitable for a wide range of biochemical assays where sterility is not required.
Specifications:
| Feature | Details |
|---|---|
| Material | Polystyrene |
| Surface Treatment | MaxiSorp, PolySorp, Immulon 1B, Immulon 2HB |
| Color | Clear |
| Well Shape | Round Bottom |
| No. of Wells | 96 |
| Volume (Metric) Well | 300 μL |
| Volume (Metric) Working | 250 μL |
| Detection Method | Colorimetric |
| Array | 8 x 12 |
| Sterility | Non-sterile |
| Plate Blocking | None |
| Target Molecules | Antibodies, Glycoproteins |
Applications:
- Solid-Phase Immunoassays:
- Designed for ELISA and other antibody-based assays requiring precise biomolecule binding.
- Protein and Biomolecule Binding:
- MaxiSorp for hydrophilic molecules and PolySorp/Immulon 1B for hydrophobic molecules.
- Colorimetric Assays:
- Clear wells ensure consistent optical properties for accurate colorimetric detection.
- Antibody Sandwich Assays:
- Optimized for high-sensitivity binding in sandwich assay configurations.
- Biomolecular Studies:
- Suitable for diverse binding studies, including glycoproteins, peptides, and antibodies.
Advantages:
- Versatile Binding Surfaces:
- Offers multiple surface treatments to support various assay requirements.
- High Reproducibility:
- Uniform well dimensions and clear labeling ensure consistency across experiments.
- Efficient Design:
- Round-bottom wells enhance mixing and reduce reagent waste.
- Convenient Handling:
- Alphanumeric labeling simplifies sample identification, even in high-throughput workflows.
Storage and Handling:
- Storage: Store in a cool, dry environment.
- Handling: Use appropriate laboratory practices to avoid contamination.
Ordering Information:
| Catalog Number | Surface Treatment | Description | Packaging |
|---|---|---|---|
| 449824 | MaxiSorp | Hydrophilic for antibody binding | 96-well plates |
| 3555TS | Immulon 1B | Hydrophobic for non-polar molecules | 96-well plates |
| 3655TS | Immulon 2HB | Slightly hydrophilic for diverse biomolecules | 96-well plates |
| 475434 | PolySorp | Hydrophobic for peptides and lipids | 96-well plates |
Applications in Research and Industry:
- Drug Discovery:
- Evaluates protein-protein and antibody interactions in preclinical research.
- Diagnostics Development:
- Develops and validates diagnostic assays using hydrophilic and hydrophobic biomolecules.
- Biochemical Assays:
- Facilitates enzyme-linked assays and colorimetric detection in clinical and research settings.
- High-Throughput Screening:
- Simplifies automation workflows for large-scale assays.
The Thermo Scientific™ Clear Round-Bottom Immuno Nonsterile 96-Well Plates provide a versatile and reliable platform for immunoassays, colorimetric assays, and protein-binding studies. Their diverse surface treatments and robust design make them essential for both research and industrial workflows.
- Surface Treatment: Immulon 1 B for Lipids Immulon 2 HB for Proteins MaxiSorp PolySorp
- Pack Size: 50/Case 60/Case
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