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Macherey-Nagel™ NucleoSpin™ Soil, Mini kit for DNA from soil
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Macherey-Nagel™ | ||
| Product Grade | Molecular Biology | ||
The Macherey-Nagel™ NucleoSpin™ Soil Kit is a specialized DNA extraction solution designed to efficiently isolate high-quality genomic DNA from a wide range of soil types, including complex and organic-rich matrices. This kit combines mechanical disruption via MN Bead Tubes Type A, chemical lysis with dual buffer options, and effective removal of PCR inhibitors using the NucleoSpin Inhibitor Removal Column.
This unique combination makes the NucleoSpin Soil kit ideal for microbial community analysis and metagenomic studies from environmental samples such as agricultural soils, composts, sediments, and sludge.
Key Features and Benefits
- Dual Lysis Buffer System: Two optimized lysis buffers target various soil types for maximum DNA yield.
- Enhanced Lysis with Bead Beating: MN Bead Tubes Type A (included) facilitate efficient mechanical disruption.
- Efficient Inhibitor Removal: Dedicated Inhibitor Removal Column eliminates humic acids and other PCR inhibitors.
- High Yield and Purity: Typical yields of 2–10 µg DNA from 500 mg soil, suitable for downstream applications like qPCR and microarrays.
- Flexible Elution Volume: DNA can be eluted in 30–100 µL based on concentration requirements.
- Wide Fragment Size Range: Isolates DNA fragments between 50 bp and ~50 kbp.
Technical Specifications
| Parameter | Specification |
|---|---|
| Application | Isolation of DNA from soil, sludge, and sediment |
| Sample Material | Soil, sludge, sediment |
| Sample Input Amount | < 500 mg |
| Fragment Size Range | 50 bp – ~50 kbp |
| Typical DNA Yield (500 mg soil) | 2 – 10 µg |
| Theoretical Binding Capacity | 50 µg DNA |
| Elution Volume | 30 – 100 µL |
| Preparation Time | Approx. 90 min (10 preps) |
| Downstream Applications | PCR, qPCR, microarrays, Southern blotting |
| Format | Mini spin column |
| Handling | Manual, centrifuge-based |
| Lysate Clarification | Centrifugation and Inhibitor Removal Column |
| Automation Compatibility | No |
| Storage and Shelf Life | 27 months from production |
| CE Certified | No (For research use only) |
| Hazardous Material | Yes (handle according to SDS) |
Available Pack Sizes
| Reference Code | Pack Size | Format |
|---|---|---|
| 740780.10 | 10 Preps | Mini prep kit |
| 740780.50 | 50 Preps | Mini prep kit |
| 740780.250 | 250 Preps | Mini prep kit |
The NucleoSpin™ Soil DNA Mini Kit from Macherey-Nagel is a trusted and versatile solution for extracting high-quality DNA from challenging environmental matrices. Its integrated mechanical and chemical lysis system, coupled with effective inhibitor removal, ensures optimal performance for downstream molecular biology applications. Whether for microbial diversity studies, qPCR, or metagenomics, this kit provides reproducible results with minimal inhibition—making it a staple in soil microbiology and environmental DNA research.
- Pack Size: For 10 Preps For 250 preps For 50 Preps