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Invitrogen™ PureLink™ Microbiome DNA Purification Kit, 50 Preps
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Sample Type; Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
Specifications:
| Application | Genomic Purification | ||
| Storage Temperature | Ambient | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The PureLink™ Microbiome DNA Purification Kit offers a comprehensive solution for extracting high-quality microbial and host DNA from diverse and challenging sample types, such as stool and soil. With its optimized triple lysis method and efficient inhibitor removal, this kit ensures robust yields of pure DNA suitable for downstream applications, including PCR, sequencing, and hybridization.
Key Features
- Efficient Triple Lysis Approach:
- Combines heat, chemical, and mechanical disruption with specialized beads to ensure lysis of all microorganisms, including those with durable cell walls (e.g., gram-positive bacteria).
- Broad Sample Compatibility:
- Effective for microbial and host DNA purification from:
- Stool
- Soil
- Urine
- Saliva
- Swabs (buccal, vaginal, skin, rectal, environmental)
- Transport and growth media
- Effective for microbial and host DNA purification from:
- Inhibitor Removal:
- Innovative cleanup buffer eliminates inhibitory compounds for enhanced DNA purity.
- Streamlined Workflow:
- Complete process takes up to 55 minutes, including sample homogenization.
- High Yield and Purity:
- Recovers up to 25 μg of DNA with minimal contamination, ensuring compatibility with qPCR, NGS, and hybridization-based applications.
Specifications
| Feature | Details |
|---|---|
| Column Type | Spin Column |
| Technology | Silica Spin Column |
| Sample Compatibility | Stool, Soil, Saliva, Swabs, Microbial Cultures, Urine |
| Scale | Mini |
| Elution Volume | 50–200 μL |
| Final Product | Microbial and Host DNA |
| Applications | PCR, qPCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| Test Time | ~55 minutes (including sample homogenization) |
| Yield | ≤25 μg DNA |
| High-Throughput Compatibility | Not compatible |
| Starting Material | Stool/Soil: ≤2 g; Urine: ≤10 mL; Swabs: 1 swab; Microbial Culture: ≤5 mL; Saliva: ≤2 mL |
| Unit Size | 1 Kit (50 Preps) |
Kit Contents
| Component | Quantity | Storage |
|---|---|---|
| S1 Lysis Buffer | 40 mL | Room temperature |
| S2 Lysis Enhancer | 5 mL | Room temperature |
| S3 Cleanup Buffer | 12.5 mL | Room temperature |
| S4 Binding Buffer | 45 mL | Room temperature |
| S5 Wash Buffer (concentrated) | 13 mL | Room temperature |
| S6 Elution Buffer | 5 mL | Room temperature |
| PureLink Columns with Collection Tubes | 50 Columns | Room temperature |
| Collection Tubes (2.0 mL) | 100 Tubes | Room temperature |
| Bead Tubes | 50 Tubes | Room temperature |
Applications
- Microbiome Research:
- Extract microbial DNA to study community composition and its role in health and disease (e.g., gut microbiota and inflammatory bowel disease).
- Environmental Analysis:
- Isolate DNA from soil or environmental swabs for ecological and metagenomic studies.
- Pathogen Detection:
- Identify and quantify pathogenic bacteria in clinical or environmental samples.
- NGS and Hybridization:
- Use the high-purity DNA for next-generation sequencing, nucleic acid labeling, or Southern blotting.
Advantages
| Feature | Benefit |
|---|---|
| Triple Lysis Technology | Ensures efficient lysis of all microorganisms, including tough species. |
| Versatile Sample Handling | Processes a wide range of biological and environmental samples. |
| Efficient Inhibitor Removal | Delivers high-purity DNA, free from contaminants. |
| Scalable Elution Volume | Adjustable elution volumes (50–200 μL) for flexible downstream applications. |
| Fast and Reliable Workflow | Complete purification in under an hour. |
Comparison with Competitor Kits
| Feature | Invitrogen PureLink Microbiome DNA Kit | Qiagen PowerSoil DNA Kit | Zymo Biomics DNA Miniprep Kit |
|---|---|---|---|
| Target DNA | Microbial and host DNA | Microbial DNA only | Microbial DNA only |
| Sample Compatibility | Stool, soil, swabs, urine, saliva | Soil, stool | Stool, saliva, swabs |
| Technology | Triple lysis with bead tubes | Bead beating | Bead beating |
| Processing Time | ~55 minutes | ~60 minutes | ~60 minutes |
| Elution Volume | 50–200 μL | 100 μL | 50–200 μL |
| Yield | ≤25 μg | ≤20 μg | ≤15 μg |
| Downstream Applications | PCR, qPCR, sequencing | PCR, qPCR, sequencing | PCR, sequencing |
| High-Throughput Compatibility | No | No | Yes |
Suggested Pairing
- Downstream Analysis:
- Pair with Ion Torrent™ NGS Platforms or Thermo Scientific™ NanoDrop Spectrophotometers for sequencing and quantification.
- Amplification:
- Use with Invitrogen Platinum™ Taq DNA Polymerase for PCR applications.
The Invitrogen™ PureLink™ Microbiome DNA Purification Kit delivers unmatched versatility and efficiency, making it the ideal choice for extracting microbial and host DNA from challenging sample types for various research applications.