Invitrogen™ Human IL-4 ELISA Kit

The Thermo Scientific™ Invitrogen™ Human IL-4 ELISA Kit is a robust, high-sensitivity assay designed for the quantitative detection of Interleukin-4 (IL-4) in human biological samples, including serum, plasma, and cell culture supernatants. IL-4 is a pleiotropic cytokine critical for immune response regulation and cell differentiation. This sandwich ELISA kit provides a reliable and specific method for quantifying IL-4 levels, making it ideal for research in immunology, inflammation, and disease mechanisms.

Key Features

1. High Sensitivity and Precision:
   • Analytical Sensitivity: <2 pg/mL.
   • Assay Range: 7.8–500 pg/mL.
   • Intraassay CV: 3%.
   • Interassay CV: 4.5%.
2. Versatile Sample Compatibility:
   • Suitable for human serum, plasma, and cell culture supernatants.
   • Requires only 100 µL of sample.
3. Comprehensive Kit Design:
   • Includes pre-coated 96-well plates, standards, and all necessary reagents for streamlined workflow.
4. Reliable Sandwich ELISA Format:
   • Ensures specific detection of natural and recombinant human IL-4 using matched antibody pairs.
5. Time-Efficient Workflow:
   • Hands-on time: 1 hr 20 min.
   • Total time-to-result: 3 hr.

Applications and Use Cases

1. Immunology Research:

• Cytokine Profiling:
  • Quantify IL-4 levels as part of broader cytokine analysis in immune response studies.
• Immune Cell Differentiation:
  • Investigate IL-4’s role in Th2 cell differentiation and B cell activation.

2. Clinical Research:

• Allergy and Asthma Studies:
  • Analyze IL-4 levels in allergic responses and inflammatory airway conditions.
• Autoimmune Diseases:
  • Explore IL-4 dysregulation in autoimmune disorders like rheumatoid arthritis and lupus.

3. Cancer Research:

• Tumor Microenvironment Analysis:
  • Study IL-4’s contribution to tumor immune evasion and cancer progression.
• Therapeutic Targeting:
  • Evaluate IL-4 as a potential target for immunotherapy.

4. Vaccine Development:

• Adjuvant Studies:
  • Monitor IL-4 production as a marker of Th2-driven immune responses following vaccination.

Kit Components

Assay Principle

The Human IL-4 ELISA Kit employs a sandwich ELISA method for accurate quantification:

1. Capture Phase:
   • A pre-coated antibody in the 96-well plate captures IL-4 from the sample.
2. Detection Phase:
   • A biotinylated detection antibody binds to a different epitope of the IL-4 molecule, forming a sandwich complex.
3. Signal Amplification:
   • Streptavidin-HRP binds to the biotinylated detection antibody, amplifying the signal.
4. Signal Development:
   • Add TMB substrate to produce a measurable color change.
5. Quantification:
   • Measure optical density (OD) at 450 nm and calculate IL-4 concentrations using the standard curve.

Performance Specifications

Storage and Shipping

• Storage Conditions: Store kit components at 2–8°C.
• Shipping Conditions: Shipped on wet ice to maintain reagent stability.

Target Information: Interleukin-4 (IL-4)

• Function:
  • A pleiotropic cytokine that regulates immune responses and cell differentiation.
  • Plays a key role in Th2-mediated immunity, B cell activation, and allergic inflammation.
• Source:
  • Produced by activated T cells as a ligand for the IL-4 receptor.
• Clinical Relevance:
  • Dysregulated IL-4 levels are implicated in allergies, asthma, autoimmune disorders, and cancer.
• Gene Information:
  • Gene Symbol: IL4 (Gene ID: 3565).
  • Aliases: BSF-1, B-cell stimulatory factor 1, Pitrakinra.
  • UniProt ID: P05112.

Advantages of the Kit

1. High Sensitivity and Specificity:
   • Detects very low levels of IL-4 with minimal cross-reactivity.
2. Validated Performance:
   • Each lot is rigorously tested for sensitivity, specificity, and reproducibility.
3. Ease of Use:
   • Ready-to-use reagents simplify setup, and pre-coated plates ensure consistent results.
4. Broad Applications:
   • Suitable for immunology, allergy research, and disease studies.

The Thermo Scientific™ Invitrogen™ Human IL-4 ELISA Kit is a reliable and sensitive assay for measuring IL-4 levels in human biological samples. Its precision, broad sample compatibility, and validated performance make it an essential tool for researchers studying immune regulation, inflammation, and cytokine signaling.