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Thermo Scientific™ Invitrogen™ Human IL-4 ELISA Kit
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Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Invitrogen™ Human IL-4 ELISA Kit is a robust, high-sensitivity assay designed for the quantitative detection of Interleukin-4 (IL-4) in human biological samples, including serum, plasma, and cell culture supernatants. IL-4 is a pleiotropic cytokine critical for immune response regulation and cell differentiation. This sandwich ELISA kit provides a reliable and specific method for quantifying IL-4 levels, making it ideal for research in immunology, inflammation, and disease mechanisms.
Key Features
- High Sensitivity and Precision:
- Analytical Sensitivity: <2 pg/mL.
- Assay Range: 7.8–500 pg/mL.
- Intraassay CV: 3%.
- Interassay CV: 4.5%.
- Versatile Sample Compatibility:
- Suitable for human serum, plasma, and cell culture supernatants.
- Requires only 100 µL of sample.
- Comprehensive Kit Design:
- Includes pre-coated 96-well plates, standards, and all necessary reagents for streamlined workflow.
- Reliable Sandwich ELISA Format:
- Ensures specific detection of natural and recombinant human IL-4 using matched antibody pairs.
- Time-Efficient Workflow:
- Hands-on time: 1 hr 20 min.
- Total time-to-result: 3 hr.
Applications and Use Cases
1. Immunology Research:
- Cytokine Profiling:
- Quantify IL-4 levels as part of broader cytokine analysis in immune response studies.
- Immune Cell Differentiation:
- Investigate IL-4’s role in Th2 cell differentiation and B cell activation.
2. Clinical Research:
- Allergy and Asthma Studies:
- Analyze IL-4 levels in allergic responses and inflammatory airway conditions.
- Autoimmune Diseases:
- Explore IL-4 dysregulation in autoimmune disorders like rheumatoid arthritis and lupus.
3. Cancer Research:
- Tumor Microenvironment Analysis:
- Study IL-4’s contribution to tumor immune evasion and cancer progression.
- Therapeutic Targeting:
- Evaluate IL-4 as a potential target for immunotherapy.
4. Vaccine Development:
- Adjuvant Studies:
- Monitor IL-4 production as a marker of Th2-driven immune responses following vaccination.
Kit Components
| Component | Description |
|---|---|
| Pre-Coated 96-Well Plate | Immobilized with a specific antibody to IL-4. |
| Standard | For generating a standard curve for quantification. |
| Standard Dilution Buffer | Optimizes sample preparation. |
| Biotinylated Detection Antibody | Specifically binds to IL-4. |
| Streptavidin-HRP | Amplifies the detection signal. |
| HRP Diluent | Ensures stable signal development. |
| Wash Buffer | Removes unbound substances. |
| Chromogen | Produces a colorimetric signal. |
| Stop Solution | Stabilizes the signal for OD measurement. |
| Adhesive Plate Covers | Prevents evaporation during incubation. |
Assay Principle
The Human IL-4 ELISA Kit employs a sandwich ELISA method for accurate quantification:
- Capture Phase:
- A pre-coated antibody in the 96-well plate captures IL-4 from the sample.
- Detection Phase:
- A biotinylated detection antibody binds to a different epitope of the IL-4 molecule, forming a sandwich complex.
- Signal Amplification:
- Streptavidin-HRP binds to the biotinylated detection antibody, amplifying the signal.
- Signal Development:
- Add TMB substrate to produce a measurable color change.
- Quantification:
- Measure optical density (OD) at 450 nm and calculate IL-4 concentrations using the standard curve.
Performance Specifications
| Feature | Details |
|---|---|
| Detection Method | Colorimetric |
| Dynamic Range | 7.8–500 pg/mL |
| Sensitivity | <2 pg/mL |
| Sample Volume Required | 100 µL |
| Species Reactivity | Human |
| Pack Size | 96 Tests |
Storage and Shipping
- Storage Conditions: Store kit components at 2–8°C.
- Shipping Conditions: Shipped on wet ice to maintain reagent stability.
Target Information: Interleukin-4 (IL-4)
- Function:
- A pleiotropic cytokine that regulates immune responses and cell differentiation.
- Plays a key role in Th2-mediated immunity, B cell activation, and allergic inflammation.
- Source:
- Produced by activated T cells as a ligand for the IL-4 receptor.
- Clinical Relevance:
- Dysregulated IL-4 levels are implicated in allergies, asthma, autoimmune disorders, and cancer.
- Gene Information:
- Gene Symbol: IL4 (Gene ID: 3565).
- Aliases: BSF-1, B-cell stimulatory factor 1, Pitrakinra.
- UniProt ID: P05112.
Advantages of the Kit
- High Sensitivity and Specificity:
- Detects very low levels of IL-4 with minimal cross-reactivity.
- Validated Performance:
- Each lot is rigorously tested for sensitivity, specificity, and reproducibility.
- Ease of Use:
- Ready-to-use reagents simplify setup, and pre-coated plates ensure consistent results.
- Broad Applications:
- Suitable for immunology, allergy research, and disease studies.
The Thermo Scientific™ Invitrogen™ Human IL-4 ELISA Kit is a reliable and sensitive assay for measuring IL-4 levels in human biological samples. Its precision, broad sample compatibility, and validated performance make it an essential tool for researchers studying immune regulation, inflammation, and cytokine signaling.