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Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit, 50 Preps
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Specifications:
| Application | PCR, Gel Electrophoresis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit provides a versatile, all-in-one solution for DNA fragment purification from agarose gels and PCR clean-up. Using a silica-based spin cartridge, the kit enables rapid, efficient purification of DNA fragments while eliminating primers, unincorporated dNTPs, enzymes, salts, and short-failed PCR products.
DNA purified with the kit is free from proteins, dyes, and agarose, and is immediately ready for downstream applications such as sequencing, cloning, in vitro transcription, restriction mapping, and labeling. With a binding capacity of up to 15 μg DNA, the kit ensures high-yield recovery with processing times of less than 30 minutes for gel extraction and under 10 minutes for PCR clean-up.
Key Features
- Dual Functionality – Combines gel extraction and PCR purification in one kit.
- High Recovery – Purifies DNA fragments from 40 bp to 10 kb with binding capacity up to 15 μg.
- Fast Protocols – <30 min for gel extraction and <10 min for PCR clean-up.
- Complete Kit – Contains all necessary buffers and Invitrogen spin columns.
- Clean DNA – Removes proteins, agarose, salts, primers, dNTPs, and enzymes.
- Flexible Downstream Applications – Compatible with sequencing, cloning, hybridization, labeling, and in vitro transcription.
Applications
- DNA fragment recovery from agarose gels.
- PCR clean-up of fragments >100 bp.
- Removal of primers, enzymes, salts, and dNTPs.
- DNA preparation for sequencing, cloning, restriction digestion, or labeling.
- Suitable for Southern blotting and nucleic acid hybridization workflows.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | DNA Purification Kit |
| Catalog Number | K220001 |
| Quantity | 50 preps |
| Sample Type | DNA fragments from agarose gels, PCR products, restriction enzyme reactions |
| Fragment Size Range | 40 bp – 10 kb (gel), >100 bp (PCR) |
| Binding Capacity | ≤15 μg DNA |
| Starting Material | ≤400 mg agarose gel, ≤100 μL PCR product, ≤40 μg dsDNA |
| Elution Volume | 50 μL |
| Test Time | Up to 30 min (gel), <10 min (PCR) |
| Technology | Silica spin column |
| Visualization | Compatible with ethidium bromide and SYBR™ Safe |
| High-Throughput | Manual use only (not automation compatible) |
| Shipping Condition | Room temperature |
| Storage Conditions | Columns recommended at 2–8 °C for long-term stability |
| Unit Size | Each kit (50 preps) |
Unique Advantages
- Combines two essential DNA purification methods in a single kit.
- Produces high-purity DNA free of inhibitors, enabling reproducible downstream results.
- Saves time with simplified protocols for both gel extraction and PCR purification.
- Trusted Invitrogen quality for sensitive molecular biology workflows.
The Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit offers researchers a flexible and efficient solution for nucleic acid purification. With its dual functionality, high recovery rates, and inhibitor-free DNA output, it streamlines laboratory workflows and ensures reliable performance in cloning, sequencing, transcription, and hybridization applications.