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Invitrogen™ Lipofectamine™ 3000 Transfection Reagent
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Specifications:
| Application | Transfection | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Lipofectamine™ 3000 Transfection Reagent represents Invitrogen’s most advanced lipid nanoparticle-based transfection technology, delivering exceptional efficiency, low cytotoxicity, and broad cell compatibility. Designed to perform across both common and hard-to-transfect cell types, it supports reliable delivery of plasmid DNA, RNA, and co-transfection experiments, including genome editing applications with CRISPR/Cas9 or TALENs.
Key Features
- Unmatched Efficiency: Highest transfection rates across more than 60 cell types, including stem cells, primary cells, and immortalized lines
- Broad Application Use: Enables DNA, RNA, and multiplex co-transfections in a single protocol
- Enhanced Cell Viability: Optimized for minimal toxicity, even at high efficiency
- Versatile Format: Compatible with various plate formats and flask scales (6- to 96-well plates, T-flasks)
- Cost-Effective: High concentration formulation means fewer microliters needed per reaction
Efficient Across a Range of Cell Types
Lipofectamine 3000 is validated in numerous cell lines:
| Cell Type | Examples |
|---|---|
| Fibroblast | NIH/3T3, COS-7 |
| Myoblast | C2C12, L6 |
| Hepatocyte | HepG2, HuH7 |
| Epithelial Carcinoma | HeLa, A549, MCF7, SK-MEL-28, Caco-2 |
| Hematopoietic | K562 |
| Osteosarcoma | U-2 OS, Saos-2 |
| Colon/Prostate | SW480, LNCap |
| Pancreatic | PANC-1 |
Genome Editing Compatibility
Lipofectamine™ 3000 improves transfection success in CRISPR and TALEN workflows by:
- Increasing nucleic acid uptake
- Boosting cleavage and recombination efficiency
- Reducing toxicity that may compromise editing fidelity
Kit Contents and Storage
- Included: Lipofectamine™ 3000 Reagent and P3000™ Enhancer Reagent
- Storage: 2–8°C (Do not freeze)
- Shelf Life: Stable for 12 months from date of manufacture under proper storage conditions
Specifications
| Attribute | Details |
|---|---|
| Product Type | Transfection Reagent |
| Transfection Method | Lipid-based nanoparticle |
| Target Molecules | Plasmid DNA, siRNA, shRNA, miRNA, mRNA |
| Application | Transfection, Co-transfection, Genome Editing |
| Cell Types | Hard-to-transfect, Stem Cells, Primary, Common Lines |
| Serum Compatibility | Yes |
| Formats Supported | 6-, 12-, 24-, 48-, 96-well plates, flasks |
| Classification | Animal Origin-Free (AOF) |
| Sustainability | Supplied in recyclable, sustainable packaging |
| Unit Sizes | 0.1 mL – 15 mL |
Lipofectamine™ 2000 vs. Lipofectamine™ 3000
| Feature | Lipofectamine™ 2000 | Lipofectamine™ 3000 |
|---|---|---|
| Transfection Efficiency | High efficiency in most common cell lines | Superior efficiency, especially in hard-to-transfect cells |
| Cell Viability | Moderate; some toxicity depending on dose | Improved cell viability with lower toxicity |
| DNA Compatibility | Excellent | Excellent |
| RNA Compatibility | siRNA, shRNA | siRNA, mRNA, miRNA, shRNA |
| Co-Transfection (DNA + RNA) | Very good | Superior performance |
| CRISPR / Genome Editing Use | Limited application | Optimized for genome editing tools (CRISPR/Cas9, TALENs) |
| Transfection Range | Effective in many cell lines | Broadest cell compatibility (including stem and primary cells) |
| Serum Compatibility | Yes; no media change needed | Yes |
| High-throughput Compatibility | Yes | Yes |
| Enhancer Reagent Included | No | Yes (P3000™ Enhancer) |
| Ease of Use | Simple 2-component protocol | Simple, slightly more optimized with enhancer step |
| Scalability | Suitable for most culture formats | Highly scalable, from microplate to flask formats |
| Packaging Sustainability | Yes | Yes |
| Recommended For | Gene expression, RNAi, stable line creation | DNA/RNA co-transfection, genome editing, hard-to-transfect cells |
Lipofectamine™ 3000 offers a breakthrough in transfection reagent technology, delivering higher transfection efficiency, greater reproducibility, and lower cytotoxicity than earlier generations. Its broad versatility and optimized performance make it the ideal solution for high-efficiency DNA, RNA, and genome editing delivery across virtually all cell types.
- Pack Size: 15 mL 0.1 mL 0.75 mL 1.5 mL 5 x 1.5 mL